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遺伝子ノックアウト細胞株 検証済

Anti-DMT1 抗体 [4C6] (ab55735)

Reviews (2)Q&A (3)References (36)
Western blot - Anti-DMT1 antibody [4C6] (ab55735)
  • Immunohistochemistry (Frozen sections) - Anti-DMT1 antibody [4C6] (ab55735)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody [4C6] (ab55735)
  • Flow Cytometry - Anti-DMT1 antibody [4C6] (ab55735)

Key features and details

  • Mouse monoclonal [4C6] to DMT1
  • Suitable for: Flow Cyt, IHC-P, IHC-Fr, WB
  • Knockout validated
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-DMT1 antibody [4C6]
    DMT1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4C6] to DMT1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, IHC-P, IHC-Fr, WBmore details

  • 種交差性

    交差種: Mouse, Human, Recombinant fragment

  • 免疫原

    Recombinant fragment corresponding to Human DMT1 aa 1-65.
    Sequence:

    MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATY FNEKISIPEEEYSCF


    Database link: P49281
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • IHC-P: Human endometrium cance Flow cyt: SH-SY5Y cells

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab55735の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.

ターゲット情報

  • 機能

    Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.

  • 組織特異性

    Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.

  • 関連疾患

    Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels.

  • 配列類似性

    Belongs to the NRAMP family.

  • 翻訳後修飾

    Ubiquitinated by WWP2.

  • 細胞内局在

    Endosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DCT 1 antibody
      • dct-1 antibody
      • DCT1 antibody
      • Divalent cation transporter 1 antibody
      • Divalent metal transporter 1 antibody
      • DMT 1 antibody
      • DMT-1 antibody
      • DMT1 antibody
      • FLJ37416 antibody
      • Natural resistance associated macrophage protein 2 antibody
      • Natural resistance-associated macrophage protein 2 antibody
      • NRAM2_HUMAN antibody
      • NRAMP 2 antibody
      • NRAMP2 antibody
      • OK/SW-cl.20 antibody
      • Slc11a2 antibody
      • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody
      • Solute carrier family 11 member 2 antibody
      see all

    画像

    • Western blot - Anti-DMT1 antibody [4C6] (ab55735)
      Western blot - Anti-DMT1 antibody [4C6] (ab55735)

      Western blot against tagged recombinant protein fragment (immunogen peptide) using ab55735 DMT1 antibody at 1ug/ml.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Frozen sections) - Anti-DMT1 antibody [4C6] (ab55735)
      Immunohistochemistry (Frozen sections) - Anti-DMT1 antibody [4C6] (ab55735)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

      IHC-Fr image of DMT1 staining on mouse duodenum using ab55735 (1:1000). The sections were fixed with paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were blocked using 10 % donkey serum for 1 hour at 24°C. ab55735 was diluted 1:1000 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and incubated with the slides at 4°C for 24 hours. The secondary antibody used was donkey polyclonal against mouse IgG conjugated to Alexa Fluor 568 (1:1000)

      This image was generated using the ascites version of the product.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody [4C6] (ab55735)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody [4C6] (ab55735)

      DMT1 antibody (ab55735) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-DMT1 antibody [4C6] (ab55735)
      Flow Cytometry - Anti-DMT1 antibody [4C6] (ab55735)

      Overlay histogram showing SH-SY5Y cells stained with ab55735 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55735, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

      This image was generated using the ascites version of the product.

    参考文献 (36)

    ab55735 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab55735 は 36 報の論文で使用されています。

    • Yang Y  et al. A Compared Study of Eicosapentaenoic Acid and Docosahexaenoic Acid in Improving Seizure-Induced Cognitive Deficiency in a Pentylenetetrazol-Kindling Young Mice Model. Mar Drugs 21:N/A (2023). PubMed: 37755077
    • Du L  et al. Inhibition of LSD1 induces ferroptosis through the ATF4-xCT pathway and shows enhanced anti-tumor effects with ferroptosis inducers in NSCLC. Cell Death Dis 14:716 (2023). PubMed: 37923740
    • Kang DY  et al. Anticancer Effects of 6-Gingerol through Downregulating Iron Transport and PD-L1 Expression in Non-Small Cell Lung Cancer Cells. Cells 12:N/A (2023). PubMed: 37998363
    • Fantin J  et al. Assessment of hypoxia and oxidative-related changes in a lung-derived brain metastasis model by [64Cu][Cu(ATSM)] PET and proteomic studies. EJNMMI Res 13:102 (2023). PubMed: 38006431
    • Wang D  et al. Loss of SLC46A1 decreases tumor iron content in hepatocellular carcinoma. Hepatol Commun 6:2914-2924 (2022). PubMed: 35811443
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DMT1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iPSC-derived microglia)
    Permeabilization
    Yes - 0.1% triton - 5 min
    Specification
    iPSC-derived microglia
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Boyd Kenkhuis

    Verified customer

    投稿 May 06 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-DMT1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (Mouse duodenum)
    Specification
    Mouse duodenum
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    投稿 Jul 16 2013

    Question

    Product code: 55735
    Inquiry: Hi, I am looking to use an anti-DMT-1 monoclonal antibody for ICC/IF. I see you have a polyclonal product which has been tested for the application. Could you please suggest a monoclonal that is likely to work? Thanks!

    Read More
    Answer

    Thank you for your email.

    We have monoclonal antibody ab55735 but it not tested in ICC/IF. https://www.abcam.com/DMT1-antibody-ab55735.html

    https://www.abcam.com/DMT1-antibody-4C6-ab117544.html

    I will be pleased to offer you testing discount code if you are interested in testing one of these in ICC/IF. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, you need to submit an Abreview for [UNTESTED SPECIES/APP] by including the code in the “Additional Comments” section so we know the Abreview is for this promotion.

    Please click the following link for more information;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Dear ：  I am a udeargraduate sutudent of neurology in china . My rearch is about DMT1(divalent metal transporter 1), which have 4 isoforms , they are isoform1(IRE) ,isoform2 (Non-IRE),isoform3(1A-IRE),isoform4(1A-Non-IRE)---- http://www.uniprot.org/uniprot/P49281#P49281. I  want to know your company product(Anti-DMT1 antibody (ab55735) )is for which isoform ?  I haven't seen this knowledges in the instruction . And do your company have other antibodys for each one of these fours isoforms? Thank you !   Expecting for your answer !                                                                                                                                            Sincerely,                                                                                                                                     

    Read More
    Answer

    Thank you for contacting us. Ab55735 will recognize all 4 isotypes of this protein in human as will ab123085. I've noticed that the isoforms differ at the C-terminal end. The immunogens for Ab55812 and ab117544 are against the N-terminal region of DMT1 so I would expect them recognize all isoforms as well. At this time do not offer products that recognize the specific isoforms of this target. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Hi! I tried using DTT versus B mercaptoethanol and boiling the top to 70 degrees. It still yielded no results. I tried combinations of using the two different loading gels and two different boiling temperatures.   I have now eliminated all suggestions you have given me so I am confident the antiobody is not working properly. I would like to get a refund and order DMT1 from another company so I do not have to stall my research. I absolutely love the TFR1 antibody from ABCAM and will continue its usage.  

    Read More
    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this DMT1 antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial. We have received no complaints about the quality of this product, therefore we are sorry that it is not working for you. I apologise for the inconvenience and am pleased to arrange for the refund in compensation as requested. Before doing so, I would just like to let you know that I can send you a free of charge replacement with any primary antibody of our catalog instead. This could be either an alternative DMT1 antibody such as 55812 or ab117544, or a vial of TFR1, if you prefer this resolution. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

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