Anti-DMPO Nitrone Adduct 抗体 (ab23702)
Key features and details
- Rabbit polyclonal to DMPO Nitrone Adduct
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
製品の概要
-
製品名
Anti-DMPO Nitrone Adduct antibody
DMPO Nitrone Adduct 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DMPO Nitrone Adduct -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.
-
特記事項
Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
-
精製度
Whole antiserum -
一次抗体 備考
Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23702の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/5000. Detects a band of approximately 17 kDa.
|
|
ELISA |
1/1000 - 1/5000.
|
特記事項 |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 17 kDa. |
ELISA
1/1000 - 1/5000. |
ターゲット情報
-
関連性
For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts. -
別名
- 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
画像
-
All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)
Lane 1 : Metmyoglobin at 3 µl
Lane 2 : Metmyoglobin-DMPO at 3 µl
Lane 3 : Metmyoglobin-DMPO at 5 µl
Lane 4 : Metmyoglobin-DMPO at 10 µl
Observed band size: 17 kDa why is the actual band size different from the predicted?
ab23702 was used at a concentration of 1/1000 or 1/5000, as noted at the top of the image.
データシートおよび資料
-
Datasheet download
参考文献 (6)
ab23702 は 6 報の論文で使用されています。
- Proniewski B et al. Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgaq*44 Mice. Front Immunol 9:938 (2018). PubMed: 29867936
- Pathak V et al. Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II. PLoS One 12:e0181732 (2017). WB ; Spinacia oleracea . PubMed: 28732060
- Kale R et al. Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A 114:2988-2993 (2017). WB . PubMed: 28265052
- Gomez-Mejiba SE et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . PubMed: 19159679
- Schildknecht S et al. Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite. FASEB J 22:215-24 (2008). WB . PubMed: 17724253
- Ramirez DC et al. Immuno-spin trapping analyses of DNA radicals. Nat Protoc 2:512-22 (2007). PubMed: 17406615