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リコンビナントRabMAb

Anti-DMC1 抗体 [EPR23207-130] (ab245217)

Reviews (1) Submit a question
Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
  • Anti-DMC1 antibody [EPR23207-130] (ab245217)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23207-130] to DMC1
  • Suitable for: WB, IHC-P, IP, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-DMC1 antibody [EPR23207-130]
    DMC1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23207-130] to DMC1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: His-tagged mouse DMC1 recombinant protein, Mouse testis, Rat testis lysates. IHC-P: Mouse testis, Rat testis tissues. IP: Mouse testis lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23207-130

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245217の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Predicted molecular weight: 38 kDa.
IHC-P
1/4000.
IP
1/30.
IHC-Fr
1/100.
特記事項
WB
1/1000. Predicted molecular weight: 38 kDa.
IHC-P
1/4000.
IP
1/30.
IHC-Fr
1/100.

ターゲット情報

  • 機能

    May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.

  • 配列類似性

    Belongs to the RecA family. DMC1 subfamily.
    Contains 1 HhH domain.

  • 細胞内局在

    Nucleus. Chromosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Disrupted meiotic cDNA 1 homolog antibody
      • Disrupted meiotic cDNA 1, yeast, homolog of antibody
      • dJ199H16.1 antibody
      • DMC 1 antibody
      • DMC1 antibody
      • DMC1 dosage suppressor of mck1 homolog antibody
      • DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
      • DMC1 homologue antibody
      • DMC1_HUMAN antibody
      • DMC1H antibody
      • DNA meiotic recombinase 1 antibody
      • HsLim15 antibody
      • LIM15 antibody
      • Meiotic recombination protein DMC1/LIM15 homolog antibody
      • MGC150472 antibody
      • MGC150473 antibody
      see all

    画像

    • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat testis lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22156371). 

      Negative control: Spleen (PMID: 21085687). 

      Exposure time: 26 seconds

    • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

      Lane 1 : His-tagged mouse DMC1 recombinant protein (aa2-340)
      Lane 2 : His-tagged mouse Rad51 recombinant protein (aa2-339)

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      No cross-reactivity with mouse Rad51 is observed. 

      Exposure time: Lane 3 seconds; Lane 2:81 seconds.

    • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      DMC1 was immunoprecipitated from 0.35 mg Mouse testis lysate 10ug with ab245217 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245217 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

      Lane 1: Mouse testis lysate 10ug

      Lane 2: ab245217 IP in Mouse testis lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245217 in Mouse testis lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse cerebrum. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

    • Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Anti-DMC1 antibody [EPR23207-130] (ab245217)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245217 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245217 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    Western blot abreview for Anti-DMC1 antibody [EPR23207-130]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Salmo salar Tissue lysate - other (Liver, mature male gonad, sperm)
    Gel Running Conditions
    Reduced Denaturing (pre-cast gel 4-15%)
    Loading amount
    20 µg
    Specification
    Liver, mature male gonad, sperm
    Blocking step
    Casein buffer as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Marie-Odile Baudement

    Verified customer

    投稿 Jul 01 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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