Anti-DLX3 抗体 (ab64953)
Key features and details
- Rabbit polyclonal to DLX3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DLX3 antibody
DLX3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DLX3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) from an internal region
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64953の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis. -
関連疾患
Defects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers. -
配列類似性
Belongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1747 Human
- Omim: 600525 Human
- SwissProt: O60479 Human
- Unigene: 134194 Human
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別名
- AI4 antibody
- Distal less homeo box 3 antibody
- DLX 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLX3 antibody (ab64953)Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64953 at 1/50 dilution. Right hand panel was also treated with immunising peptide.
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Western blot - Anti-DLX3 antibody (ab64953)All lanes : Anti-DLX3 antibody (ab64953) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab64953 は 8 報の論文で使用されています。
- Li J et al. Effects of DLX3 on the osteogenic differentiation of induced pluripotent stem cell-derived mesenchymal stem cells. Mol Med Rep 23:N/A (2021). PubMed: 33655330
- Yang G et al. BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts. Sci Rep 7:10775 (2017). ICC/IF ; Mouse . PubMed: 28883412
- Wu LA et al. Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis. J Cell Physiol 231:1189-98 (2016). WB ; Mouse . PubMed: 26595646
- Zhang Z et al. Transcriptional factor DLX3 promotes the gene expression of enamel matrix proteins during amelogenesis. PLoS One 10:e0121288 (2015). IHC-P . PubMed: 25815730
- Gahring LC et al. Nicotinic receptor Alpha7 expression during mouse adrenal gland development. PLoS One 9:e103861 (2014). IHC-FoFr ; Mouse . PubMed: 25093893
- Wu LA et al. Development and characterization of a mouse floxed Bmp2 osteoblast cell line that retains osteoblast genotype and phenotype. Cell Tissue Res 343:545-58 (2011). ICC/IF ; Mouse . PubMed: 21271257
- Wu LA et al. Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2. J Cell Physiol 225:132-9 (2010). ICC/IF ; Mouse . PubMed: 20458728
- Wu LA et al. Immortalization and characterization of mouse floxed Bmp2/4 osteoblasts. Biochem Biophys Res Commun 386:89-95 (2009). ICC/IF ; Mouse . PubMed: 19501574