Anti-DLST 抗体 (ab187699)
Key features and details
- Rabbit polyclonal to DLST
- Suitable for: IP, WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-DLST antibody
DLST 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DLST -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide within Human DLST aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001924.2)
Database link: P36957 -
ポジティブ･コントロール
- WB: HeLa, Jurkat, 293T and TCMK whole cell lysate. IP: Jurkat cell lysate. IHC-P: Human ovarian carcinoma and lung cancer tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
|
|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
1/1000.
|
| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IHC-Fr
1/1000. |
ターゲット情報
-
機能
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
パスウェイ
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6. -
配列類似性
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain. -
細胞内局在
Mitochondrion. - Information by UniProt
-
参照データベース
- Entrez Gene: 1743 Human
- Entrez Gene: 78920 Mouse
- Omim: 126063 Human
- SwissProt: P36957 Human
- SwissProt: Q9D2G2 Mouse
- Unigene: 525459 Human
- Unigene: 296221 Mouse
-
別名
- 2-oxoglutarate dehydrogenase complex component E2 antibody
- Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
- Dihydrolipoamide S succinyltransferase antibody
see all
画像
-
Western blot - Anti-DLST antibody (ab187699)All lanes : Anti-DLST antibody (ab187699) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-DLST antibody (ab187699)Detection of DLST by Western Blot of Immunprecipitate.
Lane 1: ab187699 at 1 µg/ml labeling DLST in Jurkat whole cell lysate immunoprecipitated using ab187699 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLST antibody (ab187699)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling DLST with ab187699 at a 1/1000 dilution.
-
Immunohistochemistry (Frozen sections) - Anti-DLST antibody (ab187699)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling DLST with ab187699 at a 1/1000 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab187699 は 4 報の論文で使用されています。
- Seim GL et al. Nitric oxide-driven modifications of lipoic arm inhibit α-ketoacid dehydrogenases. Nat Chem Biol 19:265-274 (2023). PubMed: 36266351
- Fiorentino TV et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378
- Dalton WB et al. Hotspot SF3B1 mutations induce metabolic reprogramming and vulnerability to serine deprivation. J Clin Invest 129:4708-4723 (2019). PubMed: 31393856
- Diaz-Muñoz MD et al. The RNA-binding protein HuR is essential for the B cell antibody response. Nat Immunol 16:415-25 (2015). WB ; Mouse . PubMed: 25706746