Anti-DLL1 抗体 (ab85346)
Key features and details
- Goat polyclonal to DLL1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DLL1 antibody
DLL1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DLL1 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Xenopus laevis, Zebrafish
-
免疫原
Synthetic peptide:
C-ATQRHLTVGEEWSQD
, corresponding to internal sequence amino acids 148-162 of Human DLL1 (NP_005609.3) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab85346 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
組織特異性
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
配列類似性
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
翻訳後修飾
Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 28514 Human
- Entrez Gene: 13388 Mouse
- Entrez Gene: 84010 Rat
- Omim: 606582 Human
- SwissProt: O00548 Human
- SwissProt: Q61483 Mouse
- SwissProt: P97677 Rat
- Unigene: 379912 Human
see all -
別名
- Delta (Drosophila) like 1 antibody
- Delta antibody
- Delta like 1 (Drosophila) antibody
see all
画像
-
Western blot - Anti-DLL1 antibody (ab85346)Anti-DLL1 antibody (ab85346) at 0.3 µg/ml + Rat Heart lysate at 35 µg
Predicted band size: 78 kDa
Observed band size: 78 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (10)
ab85346 は 10 報の論文で使用されています。
- Zhang Y et al. Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway. Environ Toxicol 38:1628-1640 (2023). PubMed: 36988346
- Gao L et al. Receptor-selective interleukin-4 mutein attenuates laser-induced choroidal neovascularization through the regulation of macrophage polarization in mice. Exp Ther Med 22:1367 (2021). PubMed: 34659513
- Banerjee A et al. Dengue virus infection impedes megakaryopoiesis in MEG-01 cells where the virus envelope protein interacts with the transcription factor TAL-1. Sci Rep 10:19587 (2020). PubMed: 33177556
- Song Y et al. The ethyl acetate extracts of radix trichosanthis protect retinal vascular endothelial cells from high glucose-induced injury. J Ethnopharmacol 240:111954 (2019). PubMed: 31085225
- Tetzlaff F et al. MPDZ promotes DLL4-induced Notch signaling during angiogenesis. Elife 7:N/A (2018). PubMed: 29620522
- Wang Z et al. Folic Acid Has a Protective Effect on Retinal Vascular Endothelial Cells against High Glucose. Molecules 23:N/A (2018). PubMed: 30213067
- Scavuzzo MA et al. Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA. Cell Rep 25:3811-3827.e7 (2018). PubMed: 30590051
- Chen KY et al. A Notch positive feedback in the intestinal stem cell niche is essential for stem cell self-renewal. Mol Syst Biol 13:927 (2017). WB . PubMed: 28455349
- Braune EB et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
- El-Kassar N et al. High levels of IL-7 cause dysregulation of thymocyte development. Int Immunol 24:661-71 (2012). PubMed: 22899673