Anti-DLC-A 抗体 - N-terminal (ab185994)
Key features and details
- Rabbit polyclonal to DLC-A - N-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DLC-A antibody - N-terminal
DLC-A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DLC-A - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human DLC-A aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_057225.2
Database link: Q9Y6G9 -
ポジティブ･コントロール
- WB: HeLa, 293T, and Jurkat whole cell lysates. IP: 293T cell lysates. IHC-P: Human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. -
配列類似性
Belongs to the dynein light intermediate chain family. -
翻訳後修飾
Phosphorylated during mitosis but not in interphase. -
細胞内局在
Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. - Information by UniProt
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参照データベース
- Entrez Gene: 101093542 Cat
- Entrez Gene: 460251 Chimpanzee
- Entrez Gene: 101154530 Cow
- Entrez Gene: 510330 Cow
- Entrez Gene: 477038 Dog
- Entrez Gene: 100716888 Guinea pig
- Entrez Gene: 51143 Human
- Entrez Gene: 235661 Mouse
see all -
別名
- Cytoplasmic dynein 1 light intermediate chain 1 antibody
- cytosolic antibody
- DC1L1_HUMAN antibody
see all
画像
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Western blot - Anti-DLC-A antibody - N-terminal (ab185994)All lanes : Anti-DLC-A antibody - N-terminal (ab185994) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody - N-terminal (ab185994)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling human breast carcinoma with ab185994 at a 1/1000 dilution.
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Immunoprecipitation - Anti-DLC-A antibody - N-terminal (ab185994)Immunoprecipitation. ab185994 at 0.1 µg/ml staining DLC-A in 293T whole cell lysate immunoprecipitated using ab185994 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: controle IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185994 は論文での使用が確認できていません。