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リコンビナントRabMAb

Anti-Dkk3 抗体 [EPR15611] (ab186409)

Submit a review Submit a question References (15)
Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Anti-Dkk3 antibody [EPR15611] (ab186409)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15611] to Dkk3
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Dkk3 antibody [EPR15611]
    Dkk3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15611] to Dkk3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal brain, heart and kidney lysates; HepG2 cells, A549 whole cell lysate. ICC/IF: HepG2 cells. IP: Human fetal brain lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15611

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186409の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
ICC/IF
1/100.
IP
1/50 - 1/70.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
ICC/IF
1/100.
IP
1/50 - 1/70.

ターゲット情報

  • 機能

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

  • 組織特異性

    Highest expression in heart, brain, and spinal cord.

  • 配列類似性

    Belongs to the dickkopf family.

  • ドメイン

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

  • 翻訳後修飾

    N- and O-glycosylated.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Dickkopf 3 antibody
      • Dickkopf 3 homolog (Xenopus laevis) antibody
      • Dickkopf 3 homolog antibody
      • Dickkopf homolog 3 antibody
      • Dickkopf related protein 3 antibody
      • Dickkopf related protein 3 Precursor antibody
      • Dickkopf WNT signaling pathway inhibitor 3 antibody
      • Dickkopf, Xenopus, homolog of, 3 antibody
      • Dickkopf-3 antibody
      • Dickkopf-related protein 3 antibody
      • Dickkopf3 antibody
      • DKK 3 antibody
      • Dkk-3 antibody
      • DKK3 antibody
      • DKK3_HUMAN antibody
      • hDkk 3 antibody
      • hDkk-3 antibody
      • hDkk3 antibody
      • Regulated in glioma antibody
      • REIC antibody
      • RIG antibody
      • RIG-like 5-6 antibody
      • RIG-like 7-1 antibody
      see all

    画像

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/1000 dilution

      Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
      Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : Human heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 38 kDa
      Observed band size: 50-70 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking buffer and concentration: 5% NFDM/TBST 

      Diluting buffer and concentration: 5% NFDM/TBST

      DKK3 shows 50-70 kDa bands, which is due to glycosylation (PMID: 28674110. PMID: 28738084). DKK3 is not expressed in PC-3 and BxPC-3. Negative control: PC-3 (PMID: 22941469), BxPC-3 (PMID: 28470144, PMID: 26395974, PMID: 34391478).

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µg

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)

      Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.

      Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Anti-Dkk3 antibody [EPR15611] (ab186409)
      Anti-Dkk3 antibody [EPR15611] (ab186409)

    参考文献 (15)

    ab186409 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186409 は 15 報の論文で使用されています。

    • Katase N  et al. DKK3/CKAP4 axis is associated with advanced stage and poorer prognosis in oral cancer. Oral Dis 29:3193-3204 (2023). PubMed: 35708905
    • Takeuchi A  et al. Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis. Cancer Res Commun 2:772-783 (2022). PubMed: 36923312
    • Liang X  et al. Dickkopf‑3 and β‑catenin play opposite roles in the Wnt/β‑catenin pathway during the abnormal subchondral bone formation of human knee osteoarthritis. Int J Mol Med 49:N/A (2022). PubMed: 35137918
    • Zhang LQ  et al. DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation. J Neuroinflammation 19:129 (2022). PubMed: 35658977
    • Katase N  et al. Establishment of anti-DKK3 peptide for the cancer control in head and neck squamous cell carcinoma (HNSCC). Cancer Cell Int 22:352 (2022). PubMed: 36376957
    View all Publications for this product

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