Anti-Dkk3 抗体 (ab187532)
Key features and details
- Rabbit polyclonal to Dkk3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dkk3 antibody
Dkk3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dkk3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Dkk3.
Database link: Q9UBP4 -
ポジティブ･コントロール
- Mouse brain extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 38 kDa.
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| IHC-P |
1/50 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 38 kDa. |
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IHC-P
1/50 - 1/200. |
ターゲット情報
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機能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
組織特異性
Highest expression in heart, brain, and spinal cord. -
配列類似性
Belongs to the dickkopf family. -
ドメイン
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 27122 Human
- Entrez Gene: 50781 Mouse
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- SwissProt: Q9QUN9 Mouse
- Unigene: 292156 Human
- Unigene: 55143 Mouse
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別名
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dkk3 antibody (ab187532)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling Dkk3 with ab187532 at 1/100. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dkk3 antibody (ab187532)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling Dkk3 with ab187532 at 1/100. Magnification: 200x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dkk3 antibody (ab187532)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Dkk3 with ab187532 at 1/100. Magnification: 200x. -
Western blot - Anti-Dkk3 antibody (ab187532)Anti-Dkk3 antibody (ab187532) at 1/500 dilution + Mouse brain extract
Predicted band size: 38 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab187532 は 10 報の論文で使用されています。
- Shareef ZA et al. DKK3's protective role in prostate cancer is partly due to the modulation of immune-related pathways. Front Immunol 14:978236 (2023). PubMed: 36845147
- Chen X et al. Single-cell and bulk tissue sequencing unravels the heterogeneity of synovial microenvironment in arthrofibrosis. iScience 26:107379 (2023). PubMed: 37705954
- Xu LZ et al. MiR-363-3p promotes prostate cancer tumor progression by targeting Dickkopf 3. J Clin Lab Anal 36:e24360 (2022). PubMed: 35303365
- El-Attar AZ et al. Vitamin D receptor polymorphism and prostate cancer prognosis. Curr Urol 16:246-255 (2022). PubMed: 36714231
- Yao J et al. lnc‑MICAL2‑1 sponges miR‑25 to regulate DKK3 expression and inhibits activation of the Wnt/β‑catenin signaling pathway in breast cancer. Int J Mol Med 49:N/A (2022). PubMed: 34970696
- Ning JZ et al. MiR-425 Promotes Migration and Invasion in Bladder Cancer by Targeting Dickkopf 3. J Cancer 11:3424-3432 (2020). PubMed: 32284738
- Peng G et al. miR-25-3p promotes glioma cell proliferation and migration by targeting FBXW7 and DKK3. Exp Ther Med 18:769-778 (2019). PubMed: 31258712
- Wu Q et al. MicroRNA-92b promotes cell proliferation and invasion in osteosarcoma by directly targeting Dickkopf-related protein 3. Exp Ther Med 15:173-181 (2018). PubMed: 29250147
- Park NS et al. Meridianin C inhibits the growth of YD-10B human tongue cancer cells through macropinocytosis and the down-regulation of Dickkopf-related protein-3. J Cell Mol Med 22:5833-5846 (2018). PubMed: 30246484
- Pan P et al. Loss of free fatty acid receptor 2 enhances colonic adenoma development and reduces the chemopreventive effects of black raspberries in ApcMin/+ mice. Carcinogenesis 38:86-93 (2017). PubMed: 27866157