Anti-DKC1/Dyskerin 抗体 (ab245559)
Key features and details
- Rabbit polyclonal to DKC1/Dyskerin
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DKC1/Dyskerin antibody
DKC1/Dyskerin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DKC1/Dyskerin -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human DKC1/Dyskerin aa 390-440. The exact sequence is proprietary. NP_001354.1
Database link: O60832 -
ポジティブ･コントロール
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245559 was affinity purified using an epitope specific to DKC1/Dyskerin immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245559の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 5-15 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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| 特記事項 |
|---|
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IP
Use at 5-15 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
ターゲット情報
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機能
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells). -
組織特異性
Ubiquitously expressed. -
関連疾患
Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. -
配列類似性
Belongs to the pseudouridine synthase TruB family.
Contains 1 PUA domain. -
細胞内局在
Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 1736 Human
- Omim: 300126 Human
- SwissProt: O60832 Human
- Unigene: 4747 Human
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別名
- CBF5 antibody
- CBF5 homolog antibody
- Cbf5p homolog antibody
see all
画像
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Western blot - Anti-DKC1/Dyskerin antibody (ab245559)All lanes : Anti-DKC1/Dyskerin antibody (ab245559) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-DKC1/Dyskerin antibody (ab245559)DKC1/Dyskerin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245559 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245559 at 1 µg/ml.
Lane 1: ab245559 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245559 は論文での使用が確認できていません。