Anti-DjC8 抗体 (ab86138)
Key features and details
- Rabbit polyclonal to DjC8
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DjC8 antibody
DjC8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DjC8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to a region between residue 203 and 253 of Human DjC8 (NP_055095.2).
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86138の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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配列類似性
Contains 1 J domain. - Information by UniProt
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参照データベース
- Entrez Gene: 22826 Human
- Entrez Gene: 68598 Mouse
- Entrez Gene: 313035 Rat
- SwissProt: O75937 Human
- SwissProt: Q6NZB0 Mouse
- SwissProt: Q642C0 Rat
- Unigene: 433540 Human
- Unigene: 29685 Mouse
see all -
別名
- DnaJ (Hsp40) homolog subfamily C member 8 antibody
- DnaJ homolog subfamily C member 8 antibody
- DNAJC 8 antibody
see all
画像
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Western blot - Anti-DjC8 antibody (ab86138)All lanes : Anti-DjC8 antibody (ab86138) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-DjC8 antibody (ab86138)Detection of DjC8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86138 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab86138 は論文での使用が確認できていません。