Anti-DIS3 抗体 (ab176802)
Key features and details
- Rabbit polyclonal to DIS3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DIS3 antibody
DIS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIS3 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
-
免疫原
Synthetic peptide within Human DIS3 aa 908-958. The exact sequence is proprietary. (NP_055768.3).
Sequence:KVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLG K
Database link: Q9Y2L1 -
ポジティブ･コントロール
- HeLa, 293T and Jurkat whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176802 was affinity purified using an epitope specific to DIS3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176802の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
|
| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 109 kDa. |
ターゲット情報
-
機能
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the RNR ribonuclease family.
Contains 1 PINc domain. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3. - Information by UniProt
-
参照データベース
- Entrez Gene: 452595 Chimpanzee
- Entrez Gene: 22894 Human
- Entrez Gene: 100442959 Orangutan
- Omim: 607533 Human
- SwissProt: Q9Y2L1 Human
- Unigene: 728935 Human
-
別名
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
see all
画像
-
Western blot - Anti-DIS3 antibody (ab176802)All lanes : Anti-DIS3 antibody (ab176802) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-DIS3 antibody (ab176802)Detection of DIS3 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176802 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab176802 は 2 報の論文で使用されています。
- Zinoviev A et al. Extraction of mRNA from Stalled Ribosomes by the Ski Complex. Mol Cell 77:1340-1349.e6 (2020). PubMed: 32006463
- Domingo-Prim J et al. EXOSC10 is required for RPA assembly and controlled DNA end resection at DNA double-strand breaks. Nat Commun 10:2135 (2019). PubMed: 31086179