Anti-DIP13B/APPL2 抗体 (ab154661)
Key features and details
- Rabbit polyclonal to DIP13B/APPL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIP13B/APPL2 antibody -
製品の詳細
Rabbit polyclonal to DIP13B/APPL2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human DIP13B/APPL2 aa 476-664 (C terminal).
Database link: Q8NEU8 -
ポジティブ･コントロール
- NT2D1 or IMR32 whole cell lysate; A431 cells; HBL435 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154661の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction. -
組織特異性
High levels in brain, heart, kidney and skeletal muscle. -
関連疾患
Note=A chromosomal aberration involving APPL2/DIP13B is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with SHANK3/PSAP2. -
配列類似性
Contains 1 PH domain.
Contains 1 PID domain. -
細胞内局在
Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. - Information by UniProt
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参照データベース
- Entrez Gene: 55198 Human
- Omim: 606231 Human
- SwissProt: Q8NEU8 Human
- Unigene: 506603 Human
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別名
- Adapter protein containing PH domain antibody
- Adapter protein containing PH domain PTB domain and leucine zipper motif 2 antibody
- adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 antibody
see all
画像
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Western blot - Anti-DIP13B/APPL2 antibody (ab154661)All lanes : Anti-DIP13B/APPL2 antibody (ab154661) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : IMR32 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DIP13B/APPL2 antibody (ab154661)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling DIP13B/APPL2 using ab154661 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIP13B/APPL2 antibody (ab154661)Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labeling DIP13B/APPL2 using ab154661 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154661 は論文での使用が確認できていません。