Anti-DIO2 抗体 (ab77779)
Key features and details
- Rabbit polyclonal to DIO2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIO2 antibody
DIO2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIO2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Goat, Cow, Pig, Xenopus laevis
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following human tissue lysates: Human Heart and Placenta, Mouse retina, P0 Whole Brain and Rat Whole Brain.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77779の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
ターゲット情報
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機能
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. -
組織特異性
Heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. -
配列類似性
Belongs to the iodothyronine deiodinase family. -
翻訳後修飾
Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 494548 Cow
- Entrez Gene: 1734 Human
- Entrez Gene: 13371 Mouse
- Entrez Gene: 414379 Pig
- Entrez Gene: 65162 Rat
- Omim: 601413 Human
- SwissProt: Q5I3B2 Cow
- SwissProt: Q92813 Human
see all -
別名
- 5DII antibody
- D2 antibody
- Deiodinase, iodothyronine, type II antibody
see all
画像
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Western blot - Anti-DIO2 antibody (ab77779)All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Additional bands at: 105 kDa, 55 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-DIO2 antibody (ab77779)All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab77779 は 9 報の論文で使用されています。
- Yu Z et al. Thyroid hormone transport and metabolism are disturbed in the placental villi of miscarriage. Reprod Biol Endocrinol 21:108 (2023). PubMed: 37968664
- Yin X et al. Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy. Cell Cycle 22:967-985 (2023). PubMed: 36710409
- Sass F et al. TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality. Mol Metab 47:101173 (2021). PubMed: 33516944
- Fischer AW et al. Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice. J Lipid Res 61:1377-1389 (2020). PubMed: 32769145
- Guilherme A et al. Control of Adipocyte Thermogenesis and Lipogenesis through ß3-Adrenergic and Thyroid Hormone Signal Integration. Cell Rep 31:107598 (2020). PubMed: 32375048
- Kim M et al. Pinealectomy increases thermogenesis and decreases lipogenesis. Mol Med Rep 22:4289-4297 (2020). PubMed: 33000192
- Olichwier A et al. Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart. Int J Mol Sci 22:N/A (2020). PubMed: 33374300
- Chen J et al. Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway. Ecotoxicol Environ Saf 173:142-148 (2019). PubMed: 30771657
- Li M et al. Loss of selenocysteine insertion sequence binding protein 2 suppresses the proliferation, migration/invasion and hormone secretion of human trophoblast cells via the PI3K/Akt and ERK signaling pathway. Placenta 55:81-89 (2017). PubMed: 28623977