Anti-DIMT1L 抗体 (ab184978)
Key features and details
- Rabbit polyclonal to DIMT1L
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIMT1L antibody
DIMT1L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIMT1L -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Cynomolgus monkey
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免疫原
Recombinant fragment corresponding to Human DIMT1L aa 1-150.
Database link: Q9UNQ2 -
ポジティブ･コントロール
- IHC-P: Human hippocampus, endothelium, and squamous epithelia tissues; WB: RPMI 8226 cell lysate; ICC: U-251 MG cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184978の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. -
配列類似性
Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 27292 Human
- Entrez Gene: 66254 Mouse
- Entrez Gene: 294718 Rat
- GenBank: NM_014473 Human
- Omim: 612499 Human
- SwissProt: Q2KHT8 Cow
- SwissProt: Q95KJ0 Cynomolgus monkey
- SwissProt: Q9UNQ2 Human
see all -
別名
- 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase antibody
- 18S rRNA dimethylase antibody
- DIM1 antibody
see all
画像
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Western blot - Anti-DIMT1L antibody (ab184978)Anti-DIMT1L antibody (ab184978) at 0.4 µg/ml + RPMI 8226 (human lymphoblast cell line) cell lysate
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (ab184978)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous epithelia tissue labelling DIMT1L with ab184978 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-DIMT1L antibody (ab184978)Immunofluorescence analysis of PFA/Triton X-100-fixed U-251 MG cells labeling DIMT1L with ab184978 at 4 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (ab184978)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling DIMT1L with ab184978 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (ab184978)Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling DIMT1L with ab184978 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab184978 は 1 報の論文で使用されています。
- Shen H et al. Structural and catalytic roles of the human 18S rRNA methyltransferases DIMT1 in ribosome assembly and translation. J Biol Chem 295:12058-12070 (2020). PubMed: 32616653