Anti-Dihydrofolate reductase (DHFR) 抗体 (ab49881)
Key features and details
- Rabbit polyclonal to Dihydrofolate reductase (DHFR)
- Suitable for: IP, IHC-P, WB
- Reacts with: Hamster, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Dihydrofolate reductase (DHFR) antibody
Dihydrofolate reductase (DHFR) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dihydrofolate reductase (DHFR) -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details -
種交差性
交差種: Hamster, Human, Recombinant fragment
交差が予測される動物種: Mouse, Rat, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human Dihydrofolate reductase (DHFR) aa 27-40 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:PLRNEFRYFQRMTT
Database link: P00374 -
ポジティブ･コントロール
- WB: Recombinant DHFR.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
特記事項（精製）
This antibody is affinity isolated. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49881の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 21 kDa.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 21 kDa. |
ターゲット情報
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機能
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. -
パスウェイ
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. -
配列類似性
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 508809 Cow
- Entrez Gene: 1719 Human
- Entrez Gene: 13361 Mouse
- Entrez Gene: 24312 Rat
- Omim: 126060 Human
- SwissProt: P00376 Cow
- SwissProt: P00374 Human
- SwissProt: P00375 Mouse
see all -
別名
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
see all
画像
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Western blot - Anti-Dihydrofolate reductase (DHFR) antibody (ab49881)All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab49881) at 0.5 µg/ml
Lane 1 : recombinant DHFR at 0.2 µg
Lane 2 : recombinant DHFR at 0.1 µg
Lane 3 : recombinant DHFR at 0.05 µg
Lane 4 : recombinant DHFR at 0.025 µg
Lane 5 : recombinant DHFR at 0.0125 µg
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab49881 は 7 報の論文で使用されています。
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746
- Nakano M et al. A-to-I RNA Editing Up-regulates Human Dihydrofolate Reductase in Breast Cancer. J Biol Chem 292:4873-4884 (2017). PubMed: 28188287
- Lee YH et al. DHFR and MDR1 upregulation is associated with chemoresistance in osteosarcoma stem-like cells. Oncol Lett 14:171-179 (2017). PubMed: 28693150
- Youn JY et al. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus. Mol Endocrinol 29:1123-33 (2015). PubMed: 26121233
- Gilbert J et al. Phase 2 trial of oxaliplatin and pemetrexed as an induction regimen in locally advanced head and neck cancer. Cancer 118:1007-13 (2012). PubMed: 21766301
- Anderson DD et al. Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J Biol Chem 287:7051-62 (2012). IP ; Human . PubMed: 22235121
- Martin SA et al. Methotrexate induces oxidative DNA damage and is selectively lethal to tumour cells with defects in the DNA mismatch repair gene MSH2. EMBO Mol Med 1:323-37 (2009). WB . PubMed: 20049736