Anti-Dihydrofolate reductase (DHFR) 抗体 (ab152159)
Key features and details
- Rabbit polyclonal to Dihydrofolate reductase (DHFR)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dihydrofolate reductase (DHFR) antibody
Dihydrofolate reductase (DHFR) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dihydrofolate reductase (DHFR) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to a region within amino acids 1-187 of Human Dihydrofolate reductase (DHFR) (Uniprot ID: P00374).
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ポジティブ･コントロール
- Human colon carcinoma tissue; A431 cells; 293T, A431, Jurkat, Raji whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. -
パスウェイ
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. -
配列類似性
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 508809 Cow
- Entrez Gene: 1719 Human
- Entrez Gene: 13361 Mouse
- Entrez Gene: 24312 Rat
- Omim: 126060 Human
- SwissProt: P00376 Cow
- SwissProt: P00374 Human
- SwissProt: P00375 Mouse
see all -
別名
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
see all
画像
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Western blot - Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab152159) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, staining Dihydrofolate reductase (DHFR) using ab152159 at a 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, staining Dihydrofolate reductase (DHFR) using ab152159 at a 1/200 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab152159 は論文での使用が確認できていません。