AB227518

Anti-Dicer 抗体

Name: Anti-Dicer 抗体 (ab227518) | Abcam
Brand: Abcam Limited
SKU: AB227518
Price: 73000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Dicer antibody

(1 Review)

(14 Publications)

Rabbit Polyclonal DICER antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DICER1.

別名を表示する

DICER, HERNA, KIAA0928, DICER1, Endoribonuclease Dicer, Helicase with RNase motif, Helicase MOI

4 Images

Supplier Data

Western blot - Anti-Dicer antibody (AB227518)

5% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab227518) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 218 kDa

true

Supplier Data

Western blot - Anti-Dicer antibody (AB227518)

5% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab227518) at 1/1000 dilution

Lane 1:

SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 30 µg

Lanes 2 - 3:

Dicer shRNA transfected SW480 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 218 kDa

true

Supplier Data

Western blot - Anti-Dicer antibody (AB227518)

5% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab227518) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lanes 2 - 3:

Dicer shRNA transfected MCF7 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 218 kDa

true

Supplier Data

Western blot - Anti-Dicer antibody (AB227518)

5% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab227518) at 1/500 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 218 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Human DICER1. The exact immunogen used to generate this antibody is proprietary information.

Q9UPY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

See full target information DICER1

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40649978

2025

Exploring the Role of Peripheral Macrophages in Glioma Progression: The Metabolic Significance of Cyclooxygenase-2 (COX-2).

Applications

Unspecified application

Species

Unspecified reactive species

Jens Pietzsch,Magali Toussaint,Cornelius Kurt Donat,Alina Doctor,Sebastian Meister,Johanna Wodtke,Markus Laube,Frank Hofheinz,Jan Rix,Winnie Deuther-Conrad,Cathleen Haase-Kohn

Science advances 11:eadt3751 PubMed40614179

2025

Dicer is cleaved by the Leader protease encoded by foot-and-mouth disease virus to promote infection in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Rodríguez-Pulido,Miguel Ángel Sanz,Lucía Camacho,Ricardo Ramos,Margarita Sáiz

MedComm 5:e475 PubMed38463393

2024

AMPK-upregulated microRNA-708 plays as a suppressor of cellular senescence and aging via downregulating disabled-2 and mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Hui Gong,Tingting Zhao,Weitong Xu,Honghan Chen,Tiepeng Li,Yu Yang,Ming Yang,Ning Huang,Chuhui Gong,Fangfang Wang,Cuiying Zhang,Jin Liu,Hengyi Xiao

Cell reports. Medicine 4:101209 PubMed37757825

2023

Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ya Zhu,Wen Yao,Xi-Sen Ni,Mu-Di Yao,Wen Bai,Tian-Jing Yang,Zi-Ran Zhang,Xiu-Miao Li,Qin Jiang,Biao Yan

International journal of molecular sciences 23: PubMed36077457

2022

Tankyrase Inhibition Attenuates Cardiac Dilatation and Dysfunction in Ischemic Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Heli Segersvärd,Juuso Siren,Sanni Perttunen,Katariina Immonen,Riikka Kosonen,Yu-Chia Chen,Johanna Tolva,Mirjami Laivuori,Mikko I Mäyränpää,Petri T Kovanen,Juha Sinisalo,Mika Laine,Ilkka Tikkanen,Päivi Lakkisto

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22412 PubMed35713587

2022

The hypoxia-induced changes in miRNA-mRNA in RNA-induced silencing complexes and HIF-2 induced miRNAs in human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adrianna Moszyńska,Maciej Jaśkiewicz,Marcin Serocki,Aleksandra Cabaj,David K Crossman,Sylwia Bartoszewska,Magdalena Gebert,Michał Dąbrowski,James F Collawn,Rafal Bartoszewski

Nature communications 12:3497 PubMed34108453

2021

Site-specific ubiquitylation acts as a regulator of linker histone H1.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Höllmüller,Simon Geigges,Marie L Niedermeier,Kai-Michael Kammer,Simon M Kienle,Daniel Rösner,Martin Scheffner,Andreas Marx,Florian Stengel

Aging 13:10275-10288 PubMed33819188

2021

CircRNA hsa_circ_0006215 promotes osteogenic differentiation of BMSCs and enhances osteogenesis-angiogenesis coupling by competitively binding to miR-942-5p and regulating RUNX2 and VEGF.

Applications

Unspecified application

Species

Unspecified reactive species

Houlin Ji,Xu Cui,Yang Yang,Xiaoxiao Zhou

International journal of molecular medicine 47: PubMed33649815

2021

circNRIP1 facilitates keloid progression via FXR1‑mediated upregulation of miR‑503‑3p and miR‑503‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Baolin Wang,Hang Yin,Hongmei Zhang,Tiantian Wang

Epigenetics 16:1317-1334 PubMed33356812

2020

Upregulation of polycistronic microRNA-143 and microRNA-145 in colonocytes suppresses colitis and inflammation-associated colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Urszula Dougherty,Reba Mustafi,Hongyan Zhu,Xiaorong Zhu,Dilip Deb,Stephen C Meredith,Fatma Ayaloglu-Butun,Michelle Fletcher,Arantxa Sanchez,Joel Pekow,Zifeng Deng,Nader Amini,Vani J Konda,Vijaya L Rao,Atsushi Sakuraba,Akushika Kwesi,Sonia S Kupfer,Alessandro Fichera,Loren Joseph,John Hart,Fang He,Tong-Chuan He,Diana West-Szymanski,Yan Chun Li,Marc Bissonnette

View all publications

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Abcam product promise

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Anti-Dicer 抗体

Anti-Dicer antibody

Western blot - Anti-Dicer antibody (AB227518)

Western blot - Anti-Dicer antibody (AB227518)

Western blot - Anti-Dicer antibody (AB227518)

Western blot - Anti-Dicer antibody (AB227518)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (14)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise