Anti-DIAPH3 抗体 (ab245659)
Key features and details
- Rabbit polyclonal to DIAPH3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIAPH3 antibody
DIAPH3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIAPH3 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human DIAPH3 aa 1-50. The exact sequence is proprietary.
Database link: Q9NSV4 -
ポジティブ･コントロール
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245659 was affinity purified using an epitope specific to DIAPH3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245659の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 137 kDa. |
ターゲット情報
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機能
Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. -
配列類似性
Belongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
ドメイン
DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction. - Information by UniProt
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参照データベース
- Entrez Gene: 81624 Human
- Omim: 614567 Human
- SwissProt: Q9NSV4 Human
- Unigene: 283127 Human
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別名
- AN antibody
- AUNA1 antibody
- Dia2 antibody
see all
画像
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Western blot - Anti-DIAPH3 antibody (ab245659)All lanes : Anti-DIAPH3 antibody (ab245659) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 137 kDa
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-DIAPH3 antibody (ab245659)DIAPH3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245659 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab245659 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245659 は論文での使用が確認できていません。