Anti-DIAPH2/DIA 抗体 (ab12319)
Key features and details
- Rabbit polyclonal to DIAPH2/DIA
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIAPH2/DIA antibody
DIAPH2/DIA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIAPH2/DIA -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide within Human DIAPH2/DIA aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60879 -
ポジティブ･コントロール
- WB: HEK-293T, HeLa and Jurkat whole cell lysates. IHC-P: Human testis tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12319の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 132 kDa.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 132 kDa. |
ターゲット情報
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機能
Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. -
組織特異性
Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes. -
関連疾患
Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
配列類似性
Belongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
発生段階
Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. -
ドメイン
DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction. -
細胞内局在
Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 1730 Human
- Entrez Gene: 100347936 Rabbit
- Omim: 300108 Human
- SwissProt: O60879 Human
- Unigene: 226483 Human
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別名
- Dia 2 antibody
- DIA antibody
- Dia drome antibody
see all
画像
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Western blot - Anti-DIAPH2/DIA antibody (ab12319)All lanes : Anti-DIAPH2/DIA antibody (ab12319) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Predicted band size: 132 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (ab12319)IHC image of ab12319 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12319, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab12319 は論文での使用が確認できていません。