AB12319

Anti-DIAPH2/DIA 抗体

Name: Anti-DIAPH2/DIA 抗体 (ab12319) | Abcam
Brand: Abcam Limited
SKU: AB12319
Price: 78000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-DIAPH2/DIA antibody

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(1 Publication)

Rabbit Polyclonal DIAPH2/DIA antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DIAPH2 aa 50-100.

別名を表示する

DIA, DIAPH2, Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (AB12319)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (AB12319)

IHC image of ab12319 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12319, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-DIAPH2/DIA antibody (AB12319)

All lanes:

Western blot - Anti-DIAPH2/DIA antibody (ab12319) at 0.1 µg/mL

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 126 kDa

false

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human DIAPH2 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O60879

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified using the peptide immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH2 also known as DIA or Diaphanous-related formin-2 is a protein that functions as a formin homology protein involved in actin polymerization. It plays an important role in the dynamic reorganization of the cytoskeleton. DIAPH2 has a molecular weight of approximately 123 kDa. Scientists have identified its expression in various tissues though it is observed significantly in the brain testis and kidney. It participates actively in bridging components of the cytoskeleton and membrane signaling complexes.

Biological function summary

DIAPH2 influences cellular processes by regulating actin filament elongation and stabilization. This protein associates with the Rho family of GTPases forming a critical complex that drives localized actin assembly. This actin polymerization is necessary for processes like cell division cell migration and maintenance of cell shape. Additionally DIAPH2 plays roles in vesicle trafficking and the formation of cellular structures like filopodia and lamellipodia linking membrane processes with cytoskeletal elements.

Pathways

DIAPH2 integrates into significant pathways such as the Rho-GTPase pathway where it acts as an effector that transduces signals from upstream activators like Rho and Rac proteins. These pathways regulate the assembly and disassembly of actin filaments critical for various cell functions. Through its interactions with other proteins such as mDia1 and ROCK DIAPH2 influences the actin cytoskeleton impacting pathways involved in cell motility and adhesion.

Researchers have linked mutations in the DIAPH2 gene with disorders like premature ovarian failure (POF) and certain types of cancer. In POF DIAPH2 disrupts normal ovarian function impacting fertility. In cancer aberrant regulation of DIAPH2 can lead to improper cell division and tumor growth. Studies have also suggested interactions with proteins such as BRCA1 indicating a potential role in pathways involved in maintaining genomic stability.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.

See full target information DIAPH2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell division 19:6 PubMed38374109

2024

Anti-cancer effects of Coix seed extract through KCTD9-mediated ubiquitination of TOP2A in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuyang Jiang,Xue Li,Chun Zhang,Jiafu Wang,Jin Li

View all publications

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIAPH2/DIA 抗体

Anti-DIAPH2/DIA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (AB12319)

Western blot - Anti-DIAPH2/DIA antibody (AB12319)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise