Anti-DHX58/RLR 抗体 (ab67270)
Key features and details
- Rabbit polyclonal to DHX58/RLR
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-DHX58/RLR antibody
DHX58/RLR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DHX58/RLR -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67270の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
ターゲット情報
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機能
Participates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower. -
配列類似性
Belongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 80861 Mouse
- Entrez Gene: 303538 Rat
- SwissProt: Q99J87 Mouse
- Unigene: 271830 Mouse
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別名
- D11LGP2 antibody
- D11lgp2e antibody
- DEXH (Asp Glu X His) box polypeptide 58 antibody
see all
画像
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Western blot - Anti-DHX58/RLR antibody (ab67270)Lane 1 : Anti-DHX58/RLR antibody (ab67270) at 1 µg/ml
Lane 2 : Anti-DHX58/RLR antibody (ab67270) at 2 µg/ml
All lanes : Rat
kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit secondary antibody
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab67270 は 6 報の論文で使用されています。
- Tan Z et al. LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities. PLoS Pathog 19:e1011620 (2023). PubMed: 37656756
- Lenoir JJ et al. Immune regulator LGP2 targets Ubc13/UBE2N to mediate widespread interference with K63 polyubiquitination and NF-κB activation. Cell Rep 37:110175 (2021). PubMed: 34965427
- Yamane D et al. Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses. Nat Microbiol 4:1096-1104 (2019). PubMed: 30988429
- Parisien JP et al. RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling. EMBO Rep 19:N/A (2018). PubMed: 29661858
- Childs KS et al. LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA. PLoS One 8:e64202 (2013). WB ; Human . PubMed: 23671710
- Childs K et al. Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86:3411-21 (2012). WB . PubMed: 22301134