Anti-DHODH 抗体 [EPR11814] (ab174288)
![Western blot - Anti-DHODH antibody [EPR11814] (ab174288)](https://www.abcam.co.jp/ps/products/174/ab174288/Images/ab174288-339731-ab174288WB1.jpg "Western blot - Anti-DHODH antibody [EPR11814] (ab174288)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11814] to DHODH
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DHODH antibody [EPR11814]
DHODH 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11814] to DHODH -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human DHODH. The exact sequence is proprietary.
Database link: Q02127 -
ポジティブ･コントロール
- WB: SKBR-3 cell lysate. Human fetal heart tissue lysate. MCF7, HepG2, Human heart, Mouse heart, Rat heart and Jurkat whole cell lysate (ab7899).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11814 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174288の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 43 kDa.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 43 kDa. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
ターゲット情報
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機能
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. -
パスウェイ
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. -
関連疾患
Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. -
配列類似性
Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. -
翻訳後修飾
The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1723 Human
- Entrez Gene: 56749 Mouse
- Entrez Gene: 65156 Rat
- Omim: 126064 Human
- SwissProt: Q02127 Human
- SwissProt: O35435 Mouse
- SwissProt: Q63707 Rat
- Unigene: 654427 Human
see all -
別名
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
see all
画像
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Western blot - Anti-DHODH antibody [EPR11814] (ab174288)All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
![Western blot - Anti-DHODH antibody [EPR11814] (ab174288)](https://www.abcam.co.jp/ps/products/174/ab174288/Images/ab174288-199805-anti-dhodh-antibody-epr11814-western-blot.jpg)
Western blot - Anti-DHODH antibody [EPR11814] (ab174288)All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDaNote: ab174288 is diluted in 1% BSA.
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![Anti-DHODH antibody [EPR11814] (ab174288)](https://www.abcam.co.jp/ps/products/174/ab174288/Images/ab174288-3-benefits-of-recombinant-antibodies.png)
Anti-DHODH antibody [EPR11814] (ab174288)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab174288 は 1 報の論文で使用されています。
- Hosseini M et al. UVB-induced DHODH upregulation, which is driven by STAT3, is a promising target for chemoprevention and combination therapy of photocarcinogenesis. Oncogenesis 8:52 (2019). PubMed: 31551419