Anti-DHHC-20 抗体 (ab110478)
Key features and details
- Rabbit polyclonal to DHHC-20
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DHHC-20 antibody -
製品の詳細
Rabbit polyclonal to DHHC-20 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human DHHC-20 (internal sequence).
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ポジティブ･コントロール
- Extracts from COLO, MCF7 and Jurkat cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab110478 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110478の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 42 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 42 kDa. |
ターゲット情報
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配列類似性
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
ドメイン
The DHHC domain is required for palmitoyltransferase activity. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 253832 Human
- Entrez Gene: 75965 Mouse
- Entrez Gene: 305923 Rat
- SwissProt: Q5W0Z9 Human
- SwissProt: Q5Y5T1 Mouse
- Unigene: 564611 Human
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別名
- 4933421L13Rik antibody
- DHHC-20 antibody
- Probable palmitoyltransferase ZDHHC20 antibody
see all
画像
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Western blot - Anti-DHHC-20 antibody (ab110478)All lanes : Anti-DHHC-20 antibody (ab110478) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from MCF7 cells
Lane 3 : Extracts from Jurkat cells
Lane 4 : Extracts from Jurkat cells with immunized peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab110478 は 1 報の論文で使用されています。
- Wang W et al. Protein Depalmitoylation Is Induced by Wnt5a and Promotes Polarized Cell Behavior. J Biol Chem 290:15707-16 (2015). PubMed: 25944911