Anti-DGKG 抗体 (ab151967)
Key features and details
- Rabbit polyclonal to DGKG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DGKG antibody
DGKG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DGKG -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to a region within amino acids 469 and 694 of Human DGKG (Uniprot ID: P49619)
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ポジティブ･コントロール
- A431, H1299, HeLa, HepG2 or Raji whole cell lysate, HeLa cells, NCIN87 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151967の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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関連性
DGKG (Diacylglycerol kinase gamma ) reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid. This enzyme initiates resynthesis of phosphoinositides consumed by phospholipase C during cellular signal transduction. DGK gamma is one of multiple DGK isozymes found in animal cells. It is expressed abundantly in the human retina and to a much lesser extent in the brain. Other tissues contain extremely low levels. -
細胞内局在
Cell Membrane and Cytoplasmic. -
参照データベース
- Entrez Gene: 1608 Human
- Entrez Gene: 110197 Mouse
- Entrez Gene: 25666 Rat
- Omim: 601854 Human
- SwissProt: P49619 Human
- SwissProt: Q2M1H4 Human
- SwissProt: Q5FWG1 Human
- SwissProt: Q91WG7 Mouse
see all -
別名
- DGKG antibody
- DAG kinase gamma antibody
- DAGK3 antibody
see all
画像
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Western blot - Anti-DGKG antibody (ab151967)Anti-DGKG antibody (ab151967) at 1/1000 dilution + H1299 lysate at 30 µg
Predicted band size: 89 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DGKG antibody (ab151967)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling DGKG with ab151967 a 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKG antibody (ab151967)Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling DGKG with ab151967 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab151967 は 1 報の論文で使用されています。
- Day P et al. Inhibitors of DAG metabolism suppress CCR2 signalling in human monocytes. Br J Pharmacol 176:2736-2749 (2019). PubMed: 31032885