Anti-DGKA 抗体 (ab64845)
Key features and details
- Rabbit polyclonal to DGKA
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DGKA antibody
DGKA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DGKA -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to internal sequence amino acids 331-370 of Human DGKA
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64845の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
ターゲット情報
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機能
Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. -
組織特異性
Lymphocytes and oligodendroglial cells. -
配列類似性
Belongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 EF-hand domains.
Contains 2 phorbol-ester/DAG-type zinc fingers. - Information by UniProt
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参照データベース
- Entrez Gene: 1606 Human
- Omim: 125855 Human
- SwissProt: P23743 Human
- Unigene: 524488 Human
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別名
- 80 kDa diacylglycerol kinase antibody
- DAG kinase alpha antibody
- DAGK antibody
see all
画像
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Western blot - Anti-DGKA antibody (ab64845)All lanes : Anti-DGKA antibody (ab64845) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DGKA antibody (ab64845)ICC/IF image of ab64845 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64845, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab64845 は 3 報の論文で使用されています。
- Velnati S et al. Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction. Front Immunol 14:1043603 (2023). PubMed: 37138877
- Jung IY et al. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res 78:4692-4703 (2018). PubMed: 29967261
- Ruffo E et al. Inhibition of diacylglycerol kinase a restores restimulation-induced cell death and reduces immunopathology in XLP-1. Sci Transl Med 8:321ra7 (2016). Human . PubMed: 26764158