Anti-DGAT1 抗体 [EPR13430-4] - N-terminal (ab181180)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DGAT1 antibody [EPR13430-4] - N-terminal
DGAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa cell lysate, HEK-293 and Jurkat whole cell lysate prepared in 1%SDS Hot lysis method Human small intestine lysates IP: Human fetal kidney lysate. ICC/IF: MCF7, HeLa cells. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13430-4 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181180の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/400.
For unpurified use at 1/70 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
1/50 - 1/100.
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IP |
1/30 - 1/50.
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WB |
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
特記事項 |
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Flow Cyt (Intra)
1/400. For unpurified use at 1/70 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50 - 1/100. |
IP
1/30 - 1/50. |
WB
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
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機能
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
パスウェイ
Lipid metabolism; glycerolipid metabolism. -
配列類似性
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8694 Human
- Omim: 604900 Human
- SwissProt: O75907 Human
- Unigene: 521954 Human
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別名
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared using RIPA lysis method
Lane 2 : HEK-293 whole cell lysate prepared using 1% SDS Hot lysis method
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate prepared using RIPA lysis method
Lane 4 : Jurkat whole cell lysate prepared using 1% SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 secondsBlocking buffer and concentration: 5% NFDM/TBST
We recommend to use 1% SDS Hot lysis method to get clear Western Blot results.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1/400 dilution (1µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab181180 (purified) at 1:30 dilution (2µg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
Lane 1 (input): Human fetal kidney lysate 10µg
Lane 2 (+): ab181180 & Human fetal kidney lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutesBlocking buffer and concentration: 5% NFDM/TBST
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Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Intracellular Flow Cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green).
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Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (8)
ab181180 は 8 報の論文で使用されています。
- Wu Z et al. Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α. Cell Commun Signal 20:25 (2022). PubMed: 35248054
- Beatty A et al. Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1. Nat Commun 12:2244 (2021). PubMed: 33854057
- Schene IF et al. Prime editing for functional repair in patient-derived disease models. Nat Commun 11:5352 (2020). PubMed: 33097693
- van Rijn JM et al. DGAT2 partially compensates for lipid-induced ER stress in human DGAT1-deficient intestinal stem cells. J Lipid Res 60:1787-1800 (2019). PubMed: 31315900
- Nardi F et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep 9:3035 (2019). PubMed: 30816200
- Che L et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). PubMed: 30985894
- van Rijn JM et al. Intestinal Failure and Aberrant Lipid Metabolism in Patients With DGAT1 Deficiency. Gastroenterology N/A:N/A (2018). WB . PubMed: 29604290
- Mitra R et al. Positive regulation of prostate cancer cell growth by lipid droplet forming and processing enzymes DGAT1 and ABHD5. BMC Cancer 17:631 (2017). PubMed: 28877685