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リコンビナントRabMAb

Anti-DGAT1 抗体 [EPR13430-4] - N-terminal (ab181180)

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Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
  • Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal
  • Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-DGAT1 antibody [EPR13430-4] - N-terminal
    DGAT1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13430-4] to DGAT1 - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, IP, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cell lysate, HEK-293 and Jurkat whole cell lysate prepared in 1%SDS Hot lysis method Human small intestine lysates IP: Human fetal kidney lysate. ICC/IF: MCF7, HeLa cells. Flow Cyt (intra): HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181180の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/400.

For unpurified use at 1/70  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
1/50 - 1/100.
IP
1/30 - 1/50.
WB
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
特記事項
Flow Cyt (Intra)
1/400.

For unpurified use at 1/70  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50 - 1/100.
IP
1/30 - 1/50.
WB
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能

    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.

  • パスウェイ

    Lipid metabolism; glycerolipid metabolism.

  • 配列類似性

    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

  • 細胞内局在

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ACAT related gene product 1 antibody
      • ACAT-related gene product 1 antibody
      • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
      • Acyl-CoA retinol O-fatty-acyltransferase antibody
      • Acyl-CoA:diacylglycerol acyltransferase antibody
      • ARAT antibody
      • ARGP1 antibody
      • C75990 antibody
      • D15Ertd23e antibody
      • Dgat antibody
      • DGAT1 antibody
      • DGAT1_HUMAN antibody
      • Diacylglycerol O acyltransferase 1 antibody
      • Diacylglycerol O-acyltransferase 1 antibody
      • DIAR7 antibody
      • Diglyceride acyltransferase antibody
      • EC 2.3.1.20 antibody
      • hCG_24006 antibody
      • MGC139064 antibody
      • Retinol O fatty acyltransferase antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      All lanes : Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared using RIPA lysis method
      Lane 2 : HEK-293 whole cell lysate prepared using 1% SDS Hot lysis method
      Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate prepared using RIPA lysis method
      Lane 4 : Jurkat whole cell lysate prepared using 1% SDS Hot lysis method

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa


      Exposure time: 3 seconds


      Blocking buffer and concentration: 5% NFDM/TBST

      We recommend to use 1% SDS Hot lysis method to get clear Western Blot results.

    • Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1/400 dilution (1µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

      ab181180 (purified) at 1:30 dilution (2µg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
      Lane 1 (input): Human fetal kidney lysate 10µg
      Lane 2 (+): ab181180 & Human fetal kidney lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa


      Exposure time: 3 minutes


      Blocking buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/50000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

      Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    • Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Western blot - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180) at 1/10000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

      Intracellular Flow Cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green). 

       

       

    • Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

      Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)
      Anti-DGAT1 antibody [EPR13430-4] - N-terminal (ab181180)

    参考文献 (8)

    ab181180 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181180 は 8 報の論文で使用されています。

    • Wu Z  et al. Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α. Cell Commun Signal 20:25 (2022). PubMed: 35248054
    • Beatty A  et al. Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1. Nat Commun 12:2244 (2021). PubMed: 33854057
    • Schene IF  et al. Prime editing for functional repair in patient-derived disease models. Nat Commun 11:5352 (2020). PubMed: 33097693
    • van Rijn JM  et al. DGAT2 partially compensates for lipid-induced ER stress in human DGAT1-deficient intestinal stem cells. J Lipid Res 60:1787-1800 (2019). PubMed: 31315900
    • Nardi F  et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep 9:3035 (2019). PubMed: 30816200
    • Che L  et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). PubMed: 30985894
    • van Rijn JM  et al. Intestinal Failure and Aberrant Lipid Metabolism in Patients With DGAT1 Deficiency. Gastroenterology N/A:N/A (2018). WB . PubMed: 29604290
    • Mitra R  et al. Positive regulation of prostate cancer cell growth by lipid droplet forming and processing enzymes DGAT1 and ABHD5. BMC Cancer 17:631 (2017). PubMed: 28877685

    レビューと Q&A

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    レビューを送る 質問を送る

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DGAT1 antibody [EPR13430-4] - N-terminal

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Retina tissue)
    Permeabilization
    Yes - triton
    Specification
    Retina tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tara Hessa

    Verified customer

    投稿 Apr 24 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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