AB201968

Anti-Desmoglein 3/PVA 抗体 [6G2C11]

Name: Anti-Desmoglein 3/PVA 抗体 [6G2C11] (ab201968) | Abcam
Brand: Abcam Limited
SKU: AB201968
Price: 74000 JPY
Availability: InStock

Anti-Desmoglein 3/PVA antibody [6G2C11]

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(1 Publication)

Mouse Monoclonal Desmoglein 3/PVA antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DSG3 aa 50-200.

別名を表示する

CDHF6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, PVA

6 Images

Flow Cytometry - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Flow cytometric analysis of A431 cells labeling Desmoglein 3/PVA with ab201968 at 1/200 dilution (green). Negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemical analysis of Paraffin-embedded Human esophageal cancer tissue labeling Desmoglein 3/PVA with ab201968 at 1/200 dilution followed by DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemical analysis of Paraffin-embedded Human esophageal tissue labeling Desmoglein 3/PVA with ab201968 at 1/200 dilution followed by DAB staining.

Supplier Data

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Human Desmoglein 3/PVA recombinant (aa55-159)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 108 kDa

false

Supplier Data

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) at 1/500 dilution

All lanes:

A431 cell lysate

Predicted band size: 108 kDa

false

Supplier Data

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

(Expected MW is 37.5 kDa)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) at 1/500 dilution

All lanes:

Human Desmoglein 3/PVA recombinant (aa55-159) protein

Predicted band size: 108 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

6G2C11

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt, IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human DSG3 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P32926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified from tissue culture supernatant.

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 3 (DSG3) also known as anti-DSG3 is an important component in maintaining cellular adhesion within epithelial tissues. It belongs to the cadherin family and has a mass of approximately 130 kDa. You will find DSG3 expressed mainly in epithelial cells particularly in the skin and mucosal linings. As a transmembrane glycoprotein DSG3 interacts with other desmoglein proteins such as desmoglein 1 to form desmosomes which are essential for the mechanical coupling of cells.

Biological function summary

This protein plays a fundamental role in cell adhesion by contributing to the integrity and strength of desmosomes. These are multi-protein complexes that secure cell-to-cell attachments in tissues exposed to mechanical stress. Within the desmosomes DSG3 interacts closely with other proteins including plakoglobin and plakophilins ensuring the stability of the cellular junctions. This makes DSG3 an integral part of maintaining tissue structure and resilience.

Pathways

Desmoglein 3 participates notably in the WNT signaling pathway and is involved in pathways associated with cell adhesion. In these pathways its interaction with plakoglobin which is also known as gamma-catenin plays an important role. This interaction influences signaling processes that modulate cellular adhesion and proliferation. Desmosomes facilitated by DSG3 are connective points in epidermal growth regulation pathways emphasizing DSG3's involvement in cellular cohesion and communication.

Desmoglein 3 is strongly linked to the autoimmune disease pemphigus vulgaris. In this condition autoantibodies target DSG3 disrupting its function in desmosomes and leading to blistering of the skin and mucous membranes. Desmoglein 3's relationship with desmoglein 1 is also relevant in the disease process as these proteins' shared function in cell adhesion becomes compromised. Therefore understanding DSG3's interactions and functions is vital for exploring therapeutic strategies in treating such disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 31835537). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (PubMed : 31835537). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed : 9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (PubMed : 22294297). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (PubMed : 31835537). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (PubMed : 31835537). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (PubMed : 22796473). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (PubMed : 22294297). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed : 26763450).

See full target information DSG3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1871 PubMed39984440

2025

Activation of CAMK2 by pseudokinase PEAK1 represents a targetable pathway in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yang,Xiuquan Ma,Tianyue Zhao,David R Croucher,Elizabeth V Nguyen,Kimberley C Clark,Changyuan Hu,Sharissa L Latham,Charles Bayly-Jones,Bao V Nguyen,Srikanth Budnar,Sung-Young Shin,Lan K Nguyen,Thomas R Cotton,Anderly C Chüeh,Terry C C Lim Kam Sian,Margaret M Stratton,Andrew M Ellisdon,Roger J Daly

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 3/PVA 抗体 [6G2C11]

Anti-Desmoglein 3/PVA antibody [6G2C11]

Flow Cytometry - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise