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リコンビナントRabMAb

Anti-Desmoglein 1/DSG1 抗体 [EPR20382] (ab209490)

Submit a review Submit a question References (1)
Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
  • Multiplex immunohistochemistry - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
  • Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1
  • Suitable for: WB, IHC-P, mIHC
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Desmoglein 1/DSG1 antibody [EPR20382]
    Desmoglein 1/DSG1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, mIHCmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human skin tissue lysate. IHC-P: Human esophagus and skin tissue. mIHC: Human skin (epidermis).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab209490の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 114 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
mIHC
1/1000.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 114 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
mIHC
1/1000.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

  • 組織特異性

    Epidermis, tongue, tonsil and esophagus.

  • 関連疾患

    Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.

  • 配列類似性

    Contains 4 cadherin domains.

  • ドメイン

    Calcium may be bound by the cadherin-like repeats.

  • 細胞内局在

    Cell membrane. Cell junction > desmosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    画像

    • Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
      Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
      All lanes : Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490) at 1/5000 dilution

      Lane 1 : Human skin lysates prepared in RIPA lysis method
      Lane 2 : Human skin lysates prepared in 1%SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

      Predicted band size: 114 kDa



      The expression profile and molecular weight observed are consistent with what have been described in the literature (PMID: 10634593, 29192645)

      Observed MW: 150kDa

      Block/diluting buffer and concentration: 5% NFDM/TBST

       

    • Multiplex immunohistochemistry - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
      Multiplex immunohistochemistry - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

      Panel A: merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin.
      Panel B: anti-TRP2/DCT stained on melanocytes.
      Panel C: anti-Langerin stained on Langerhans cells.
      Panel D: anti-Desmoglein 1/DSG1 stained on epidermis.

      The section was incubated in three rounds of staining: in the order of ab236259, ab283686, and ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human skin (PMID 15606502) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

      Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

      Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous staining on human esophagus (PMID: 24220297) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

      Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)
      Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab209490 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab209490 は 1 報の論文で使用されています。

    • Kleuskens MTA  et al. Butyrate and propionate restore interleukin 13-compromised esophageal epithelial barrier function. Allergy N/A:N/A (2021). PubMed: 34458999

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