Anti-Desmoglein 1/DSG1 抗体 [EPR6766(B)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] - BSA and Azide free (AB228343)

ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in paraffin embedded human skin tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124798).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] - BSA and Azide free (AB228343)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124798). Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control. We recommend using 1%SDS Hot lysis prepare method to get desired Western Blot results.

All lanes:

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (<a href='/products/primary-antibodies/desmoglein-1-dsg1-antibody-epr6766b-ab124798'>ab124798</a>) at 1/10000 dilution

Lane 1:

Human skin lysate prepared in RIPA lysis method at 20 µg

Lane 2:

Human skin lysate prepared in 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 114 kDa

Observed band size: 160 kDa

false

Exposure time: 20s