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Anti-Desmin 抗体 [RD301] (ab8976)

Reviews (1)Q&A (4)References (14)
Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [RD301] (ab8976)
  • Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [RD301] (ab8976)

Key features and details

  • Mouse monoclonal [RD301] to Desmin
  • Suitable for: IHC-Fr
  • Reacts with: Human, Zebrafish
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-Desmin antibody [RD301]
    Desmin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [RD301] to Desmin

  • 由来種

    Mouse

  • 特異性

    This antibody reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.

  • アプリケーション

    適用あり: IHC-Frmore details

  • 種交差性

    交差種: Human, Zebrafish

  • 免疫原

    Tissue, cells or virus corresponding to Chicken Desmin. Cytoskeletal desmin extract of chicken gizzard.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8976の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/100 - 1/2000. with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
特記事項
IHC-Fr
1/100 - 1/2000. with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

ターゲット情報

  • 機能

    Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.

  • 関連疾患

    Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [RD301] (ab8976)
      Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [RD301] (ab8976)

      Fluorescence microscopy of (a) cultured BHK-21 cells, (b) human skeletal muscle and (c) humanmyocardium stained with the monoclonal desmin antibody ab8976.

    • Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [RD301] (ab8976)
      Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [RD301] (ab8976)
      Immunofluorescence staining of a 7 days old zebrafish embryo

    参考文献 (14)

    ab8976 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8976 は 14 報の論文で使用されています。

    • Matsumura G  et al. Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model. PLoS One 17:e0267274 (2022). PubMed: 35436313
    • Ji G  et al. Case Report: A Novel Splice-Site Mutation in DNAJB6 Associated With Juvenile-Onset Proximal-Distal Myopathy in a Chinese Patient. Front Genet 13:925926 (2022). PubMed: 35812750
    • Zhang XZ  et al. Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model. Bioact Mater 6:1827-1838 (2021). PubMed: 33336114
    • Goto O  et al. Novel Method of Sampling the Gastrointestinal Muscle Layer: Feasibility of Endoscopic Muscular Resection with a Ligation Device in an in Vivo Porcine Model. J Nippon Med Sch 88:441-447 (2021). PubMed: 34789604
    • Luca E  et al. Genetic deletion of microRNA biogenesis in muscle cells reveals a hierarchical non-clustered network that controls focal adhesion signaling during muscle regeneration. Mol Metab 36:100967 (2020). PubMed: 32240622
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [RD301]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Carotid Artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Carotid Artery
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Le Zhen

    Verified customer

    投稿 Aug 22 2019

    Question

    I'm sorry for the belated reply. To answer your question. Yes, the old tube lot 225987 is not working. I hope that answers all of questions.

    Read More
    Answer

    Thank you for clarifying this information. As you have had good results with the other lot I believe the problems is with the vial you received and I would be happy to offer you a replacement vial if the antibody was purchased in the last 120 days. If you could please provide your order details I can arrange a free order for you, I look forward to hearing from you,

    Read More

    Question

    BATCH NUMBER 22587 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM we did immunhistos with two of your lots. lot number 25677 gave a very nice Z-disc and intercalated disc staining. we used ist 1:200. Lot number 22587 did not give any signal at all. Two different persons used it at dilution 1:100 and 1:200. SAMPLE Mouse heart, frozen sections. PRIMARY ANTIBODY Desmin (see above) DETECTION METHOD Fluorescence microscop POSITIVE AND NEGATIVE CONTROLS USED as negative control we used only the second antibody. As positive control we used a second antibody in order to colocalize it with desmin. That worked.ANTIBODY STORAGE CONDITIONS -20°C FIXATION OF SAMPLE we used both : actone (-20°C) and Paraformaldehyde. It did not work on either sections. The other lot worked with acetone fixation. BLOCKING CONDITIONS BSA 2% in PBS for 1 hour SECONDARY ANTIBODY FITC anti-mouse Vector HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? We used different dilution and fixation methods. But after finding out that lot number 25677 works we did not do more tests. ADDITIONAL NOTES I could send you the pictures I have taken that have worked. In this case please just write me an email.

    Read More
    Answer

    Thank you for contacting us for technical support regarding your recent problem with a vial of ab8976. I'm sorry to hear you are experiencing problems with this vial and I would appreciate if you could please clarify which vial is not working for you. According to your orders you have received two tubes: lot 256477 is the most recent one (the one you said worked well) was ordered a month ago lot 225987 was ordered in December 2006 (the one you said did not work). Is this correct or is it the opposite happening i.e. the new vial is not working? Thank you very much for clarifying, I look forward to hearing from you to resolve this matter,

    Read More

    Question

    What dilutions of ab8976 have you tried? 1:500, 1:400, 1:300 Do you know that the secondary antibody is working properly? Yes, we used them to detect alpha actin, GAPDH and some other antibodies in the same days/same membranes. Also, did you ensure that the protein transferred properly to the membrane? Yes, we detect GAPDH on the same membrane. Thank you again, and i look forward to hearing from you.

    Read More
    Answer

    Thank you for your email. I would expect for you to detect desmin in your samples, smooth muscle cells, and you certainly have tried high enough concentrations of ab8976. How long did you incubate with the primary? I would suggest incubating overnight at 4C. Other than that, your protocol looks fine to me. We have not had any other complaints regarding this product, and there is a possibilty that the vial you received had gone off during the shipping process. I can offer to send you a free of charge replacement vial of ab8976 or can offer you a credit note or refund. Please let me know which you would prefer.

    Read More

    Question

    BATCH NUMBER 124239 ORDER NUMBER 92612 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Smooth muscle cells (human and mouse), Mouse aorta tissue extract DETECTION METHOD Pierce SuperSignal West Piko Chemiluminescent Substrate POSITIVE AND NEGATIVE CONTROLS USED Control for secondary antibodies - used primary anti human/mouse GAPDH ANTIBODY STORAGE CONDITIONS -80 C SAMPLE PREPARATION RIPA + protease inhibitor coctail AMOUNT OF PROTEIN LOADED 30 ug ELECTROPHORESIS/GEL CONDITIONS Reduced 8% gel TRANSFER AND BLOCKING CONDITIONS Tris/Glycin buffer + 10% Methanol Tris buffer + 5% Milk (no fat) SECONDARY ANTIBODY Pierce, anti mouse, 1:10000, 1:5000, 1:4000 1,5 hour Tris buffer + 5% Milk (no fat) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

    Read More
    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab8976. Can you please provide me with a few additional details: What dilutions of ab8976 have you tried? Do you know that the secondary antibody is working properly? Also, did you ensure that the protein transferred properly to the membrane? Thank you again, and i look forward to hearing from you.

    Read More

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