Anti-Desmin 抗体 (ab8592)
Key features and details
- Rabbit polyclonal to Desmin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Desmin antibody
Desmin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Desmin -
由来種
Rabbit -
特異性
In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas. -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Human Desmin.
Database link: P17661 -
特記事項
Prolonged fixation in buffered formalin can destroy the epitope.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.01% PBS, 0.2% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8592の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa. |
追加情報
Is unsuitable for ICC/IF or IHC-P.
ターゲット情報
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機能
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
関連疾患
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
配列類似性
Belongs to the intermediate filament family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
see all -
別名
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
画像
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Western blot - Anti-Desmin antibody (ab8592)All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution
Lane 1 : RMS13 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human heart tissue lysate
Lane 5 : C2C12 cell lysate
Lane 6 : Mouse skeletal muscle tissue lysate
Lane 7 : Mouse heart tissue lysate
Lane 8 : L6 cell lysate
Lane 9 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (103)
ab8592 は 103 報の論文で使用されています。
- Wilhelmsen A et al. Skeletal muscle myostatin mRNA expression is upregulated in aged human adults with excess adiposity but is not associated with insulin resistance and ageing. Geroscience 46:2033-2049 (2024). PubMed: 37801203
- Tyganov SA et al. Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats. Int J Mol Sci 24:N/A (2023). PubMed: 36835551
- Mastrototaro G et al. Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities. Elife 12:N/A (2023). PubMed: 36927816
- Fontelonga T et al. Tetraspanin CD82 Associates with Trafficking Vesicle in Muscle Cells and Binds to Dysferlin and Myoferlin. Adv Biol (Weinh) 7:e2300157 (2023). PubMed: 37434585
- Esper ME et al. Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease. Methods Mol Biol 2566:113-132 (2023). PubMed: 36152246