Anti-DEPDC5 抗体 (ab185565)
Key features and details
- Rabbit polyclonal to DEPDC5
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DEPDC5 antibody
DEPDC5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DEPDC5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to Human DEPDC5 aa 450-550.
Database link: O75140 -
ポジティブ･コントロール
- Human stomach tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185565の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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配列類似性
Belongs to the IML1 family.
Contains 1 DEP domain. - Information by UniProt
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参照データベース
- Entrez Gene: 9681 Human
- Entrez Gene: 277854 Mouse
- Omim: 614191 Human
- SwissProt: O75140 Human
- SwissProt: P61460 Mouse
- Unigene: 435022 Human
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別名
- DEP domain containing 5 antibody
- DEP domain-containing protein 5 antibody
- DEP.5 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DEPDC5 antibody (ab185565)
Ab185565 Immunofluorescent staining of human cell line HeLa shows positivity in the Golgi apparatus. Antibody staining is shown in green.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab185565 は 5 報の論文で使用されています。
- Bacq A et al. Cardiac Investigations in Sudden Unexpected Death in DEPDC5-Related Epilepsy. Ann Neurol 91:101-116 (2022). PubMed: 34693554
- Figlia G et al. Brain-enriched RagB isoforms regulate the dynamics of mTORC1 activity through GATOR1 inhibition. Nat Cell Biol 24:1407-1421 (2022). PubMed: 36097071
- Ishida S et al. Dorsal telencephalon-specific Nprl2- and Nprl3-knockout mice: novel mouse models for GATORopathy. Hum Mol Genet 31:1519-1530 (2022). PubMed: 34965576
- De Fusco A et al. Acute knockdown of Depdc5 leads to synaptic defects in mTOR-related epileptogenesis. Neurobiol Dis 139:104822 (2020). PubMed: 32113911
- Graber TG et al. Skeletal muscle-specific knockout of DEP domain containing 5 protein increases mTORC1 signaling, muscle cell hypertrophy, and mitochondrial respiration. J Biol Chem 294:4091-4102 (2019). PubMed: 30635399