Anti-DENND1B 抗体 (ab187903)
Key features and details
- Rabbit polyclonal to DENND1B
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DENND1B antibody -
製品の詳細
Rabbit polyclonal to DENND1B -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human DENND1B aa 600-700. Isoform 5.
Database link: Q6P3S1-5 -
ポジティブ･コントロール
- IHC-P: Human colon tissue; ICC: U-2 OS whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187903の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes. -
組織特異性
Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells. -
配列類似性
Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain. -
翻訳後修飾
Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652. -
細胞内局在
Cytoplasm > cytosol. Cytoplasmic vesicle > clathrin-coated vesicle. - Information by UniProt
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参照データベース
- Entrez Gene: 163486 Human
- Omim: 613292 Human
- SwissProt: Q6P3S1 Human
- Unigene: 125056 Human
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別名
- C1ORF18 antibody
- C1orf218 antibody
- Chromosome 1 open reading frame 218 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENND1B antibody (ab187903)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling DENND1B in the cytoplasm of glandular cells with ab187903 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-DENND1B antibody (ab187903)Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling DENND1B in the nucleus speckles and cytosol with ab187903 at 2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab187903 は論文での使用が確認できていません。