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Anti-Dengue Virus prM glycoprotein 抗体 [DM1] (ab41473)

Submit a review Q&A (3)References (9)
Western blot - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Key features and details

  • Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein
  • Suitable for: ICC/IF, WB, ELISA
  • Reacts with: Dengue virus 2
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Dengue Virus prM glycoprotein antibody [DM1]
    Dengue Virus prM glycoprotein 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.

  • アプリケーション

    適用あり: ICC/IF, WB, ELISAmore details

  • 種交差性

    交差種: Dengue virus 2

  • 免疫原

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Proclin 150
    Constituents: 10% BSA, 89.9% RPMI 1640
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    DM1

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab41473の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/5 - 1/20.
WB
1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA
Use at an assay dependent dilution.
特記事項
ICC/IF
1/5 - 1/20.
WB
1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA
Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    There are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane.

  • 別名

    • pre membrane glycoprotein antibody
    • pre-membrane glycoprotein antibody

画像

  • Western blot - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    Western blot - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

    Lanes 1-2 : Dengue Virus 1 infected C6/36 cell lysate
    Lanes 3-4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
    Lanes 5-6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
    Lanes 7-8 : Dengue Virus 3 infected C6/36 cell lysate
    Lanes 9-10 : Dengue Virus 4 infected C6/36 cell lysate

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
    Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).

参考文献 (9)

ab41473 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab41473 は 9 報の論文で使用されています。

  • Jiang L & Sun Q The Role of Autophagy-Mediated Dengue Virus Antibody-Dependent Enhancement Infection of THP-1 Cells. Intervirology 63:57-65 (2020). PubMed: 33202415
  • Cortese M  et al. Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production. Cell Rep 27:2579-2592.e6 (2019). PubMed: 31141684
  • Xie X  et al. Dengue NS2A Protein Orchestrates Virus Assembly. Cell Host Microbe 26:606-622.e8 (2019). PubMed: 31631053
  • Saisawang C  et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13:e0193133 (2018). PubMed: 29470500
  • Jiang L & Sun Q The expression profile of human peripheral blood mononuclear cell miRNA is altered by antibody-dependent enhancement of infection with dengue virus serotype 3. Virol J 15:50 (2018). PubMed: 29566761
  • Huang X  et al. Antibody-dependent enhancement of dengue virus infection inhibits RLR-mediated Type-I IFN-independent signalling through upregulation of cellular autophagy. Sci Rep 6:22303 (2016). PubMed: 26923481
  • Palma-Ocampo HK  et al. Interferon lambda inhibits dengue virus replication in epithelial cells. Virol J 12:150 (2015). ICC/IF . PubMed: 26411318
  • Décembre E  et al. Sensing of immature particles produced by dengue virus infected cells induces an antiviral response by plasmacytoid dendritic cells. PLoS Pathog 10:e1004434 (2014). PubMed: 25340500
  • Liang Z  et al. Activation of Toll-like receptor 3 impairs the dengue virus serotype 2 replication through induction of IFN-ß in cultured hepatoma cells. PLoS One 6:e23346 (2011). ICC/IF ; Dengue virus 2 . PubMed: 21829730

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

I have a few quesitons regarding this antibody.

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Answer

Q1. What cell type was infected with the virus in order to produce the immunogen?
A1: The mosquito cell line, C6/36, is used for growing virus.

Q2. How was the immunogen purified?
A2: Infected supernatant and lysate were used as immunogen. No purified protein was used.

Q3. Does this antibody bind to the PR peptide or to the mature protein?
A3: Sorry but we do not have any information on the specific epitope recognized by this clone. We do know that it seems the conformational epitope is mostly lost in the western blot which likely explains why DM1 reacts to all 4 dengue serotypes in a sandwich ELISA but only to dengue 2 in the western blot.


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Question

question sur ab41473.

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Answer



J’ai contacté le laboratoire qui m’a envoyé les résultants ELISA, voir pièce jointe.

Pour le western blot, les lysats de cellules C6/36 infectés ont été dilués dans un tampon non réducteur contenant de l’SDS-PAGE. Les échantillons n’ont pas été chauffés afin de minimiser la perte d'épitopes conformationnels.

Nous pensons que la quantité de prM dans la préparation du lysat peut être un problème ici et que la seule façon de confirmer la réactivité contre tous les 4 sérotypes de la dengue serait d'utiliser des protéines recombinantes prM correctement repliées (que nous n'avons pas).

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Question

Dear Sir. or Madam I try to order the anti-prM antibody (Code:ab41473). I try to make sure whether the antibody cross react with DENV1, 3, 4? The datasheet reads it recognize DENV 1,2,3,4, but the WB pricture shows it only react with DENV2. Thanks Best

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Answer

Thank you for your enquiry. This antibody recognizes all 4 serotypes in ELISA, however in Western blot application it detects only Dengue 2. I hope this information will be useful for you.

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