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Anti-delta Sarcoglycan 抗体 (ab115019)

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Western blot - Anti-delta Sarcoglycan antibody (ab115019)

    Key features and details

    • Goat polyclonal to delta Sarcoglycan
    • Suitable for: IHC-P, WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-delta Sarcoglycan antibody
      delta Sarcoglycan 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to delta Sarcoglycan

    • 由来種

      Goat

    • アプリケーション

      適用あり: IHC-P, WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Cow, Dog, Pig

    • 免疫原

      Synthetic peptide:

      C-RLPHGSYTPTGTR

      , corresponding to internal sequence amino acids 245-257 of Human delta Sarcoglycan(NP_000328.2; NP_758447.1; NP_001121681.1).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Human and Rat heart lysates

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab115019の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).

    1 hour primary incubation is recommended for this product.
    特記事項
    IHC-P
    Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).

    1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

    • 組織特異性

      Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.

    • 関連疾患

      Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
      Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

    • 配列類似性

      Belongs to the sarcoglycan beta/delta/gamma/zeta family.

    • 翻訳後修飾

      Glycosylated.
      Disulfide bonds are present.

    • 細胞内局在

      Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 35 kDa dystrophin associated glycoprotein antibody
        • 35 kDa dystrophin-associated glycoprotein antibody
        • 35DAG antibody
        • CMD1L antibody
        • DAGD antibody
        • Delta-sarcoglycan antibody
        • Delta-SG antibody
        • Dystrophin associated glycoprotein delta sarcoglycan antibody
        • LGMD2F antibody
        • MGC22567 antibody
        • Placental delta sarcoglycan antibody
        • Sarcoglycan delta (35 kDa dystrophin associated glycoprotein) antibody
        • SG delta antibody
        • SGCD antibody
        • SGCD_HUMAN antibody
        • SGCDP antibody
        • SGD antibody
        see all

      画像

      • Western blot - Anti-delta Sarcoglycan antibody (ab115019)
        Western blot - Anti-delta Sarcoglycan antibody (ab115019)
        Anti-delta Sarcoglycan antibody (ab115019) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

        Developed using the ECL technique.

        Predicted band size: 32 kDa
        Observed band size: 37 kDa why is the actual band size different from the predicted?


        Exposure time: 1 hour

      参考文献 (0)

      ab115019 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab115019 は論文での使用が確認できていません。

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