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リコンビナントRabMAb

Anti-Delta Opioid Receptor 抗体 [EPR5029(2)] - BSA and Azide free (ab246339)

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Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
  • Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free
    Delta Opioid Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human brain, Mouse spleen, Rat spleen, and Mouse brain lysates; ICC/IF: SH-SY5Y cells. Flow Cyt (intra): U-87 MG cells.

  • 特記事項

    ab246339 is the carrier-free version of ab176324.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246339の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
ICC/IF
1/100 - 1/500.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
ICC/IF
1/100 - 1/500.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 関連性

    Function: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.

  • 細胞内局在

    Multi pass membrane protein

  • 参照データベース

    see all

  • 別名

    • D-OR-1 antibody
    • Delta type opioid receptor antibody
    • Delta type opioid receptor DOR 1 antibody
    • Delta type opioid receptor DOR1 antibody
    • Delta-type opioid receptor antibody
    • DOR 1 antibody
    • DOR-1 antibody
    • DOR1 antibody
    • mDOR antibody
    • Nbor antibody
    • Opioid receptor delta 1 antibody
    • OPRD 1 antibody
    • OPRD antibody
    • OPRD_HUMAN antibody
    • Oprd1 antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
    Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)

    Intracellular Flow Cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324)

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
    Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/100 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324)
  • Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
    Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)

    Intracellular flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red)or a rabbit IgGcontrol (green). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
    Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)

    Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

    Control: primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324).

  • Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)
    Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (ab246339)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab246339 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab246339 は論文での使用が確認できていません。

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