Anti-Delta-catenin 抗体 [EPR17628] (ab184917)
![Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (ab184917)](https://www.abcam.co.jp/ps/products/184/ab184917/Images/ab184917-244703-anti-delta-2-catenin-antibody-epr17628-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (ab184917)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17628] to Delta-catenin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-Delta-catenin antibody [EPR17628]
Delta-catenin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17628] to Delta-catenin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse brain, hippocampus and cerebellum; and rat brain and hippocampus tissue lysates. PC-12 whole cell lysate. ICC/IF: Neuro-2a cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17628 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184917の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa).
|
| ICC/IF | (1) |
1/500.
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa). |
|
ICC/IF
1/500. |
ターゲット情報
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機能
Functions as a transcriptional activator when bound to ZBTB33 (By similarity). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules. -
組織特異性
Predominantly expressed in brain. -
配列類似性
Belongs to the beta-catenin family.
Contains 9 ARM repeats. -
翻訳後修飾
O-glycosylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cell junction > adherens junction. - Information by UniProt
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参照データベース
- Entrez Gene: 18163 Mouse
- Entrez Gene: 114028 Rat
- SwissProt: O35927 Mouse
- SwissProt: O35116 Rat
- Unigene: 321648 Mouse
- Unigene: 15163 Rat
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別名
- ARM-repeat protein antibody
- Catenin (cadherin associated protein) delta 2 antibody
- catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (ab184917)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling delta-catenin with ab184917 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and nuclear staining on Neuro-2a cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184917 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. -
![Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)](https://www.abcam.co.jp/ps/products/184/ab184917/Images/ab184917-237961-anti-delta-2-catenin-antibody-epr17628-western-blot.jpg)
Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 10 seconds
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![Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)](https://www.abcam.co.jp/ps/products/184/ab184917/Images/ab184917-237960-anti-delta-2-catenin-antibody-epr17628-western-blot.jpg)
Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution
Lane 1 : Rat hippocampus lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 3 minutes
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![Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)](https://www.abcam.co.jp/ps/products/184/ab184917/Images/ab184917-237959-anti-delta-2-catenin-antibody-epr17628-western-blot.jpg)
Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution
Lane 1 : Mouse hippocampus lysate
Lane 2 : Mouse cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 10 seconds
-
![Anti-Delta-catenin antibody [EPR17628] (ab184917)](https://www.abcam.co.jp/ps/products/184/ab184917/Images/ab184917-6-benefits-of-recombinant-antibodies.png)
Anti-Delta-catenin antibody [EPR17628] (ab184917)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab184917 は 3 報の論文で使用されています。
- Tan CX et al. δ-Catenin controls astrocyte morphogenesis via layer-specific astrocyte-neuron cadherin interactions. J Cell Biol 222:N/A (2023). PubMed: 37707499
- Hu Y et al. Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway. Front Genet 13:867872 (2022). PubMed: 36303547
- Yang X et al. Hypoxia-induced lncRNA EIF3J-AS1 accelerates hepatocellular carcinoma progression via targeting miR-122-5p/CTNND2 axis. Biochem Biophys Res Commun 518:239-245 (2019). PubMed: 31421822