Anti-delta 1 Catenin/CAS (phospho T916) 抗体 [EPR2383] (ab79400)
![Western blot - Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)](https://www.abcam.co.jp/ps/products/79/ab79400/Images/ab79400-94954-anti-delta-1-catenin-cas-phospho-t916-antibody-epr2383-western-blot.jpg "Western blot - Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2383] to delta 1 Catenin/CAS (phospho T916)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383]
delta 1 Catenin/CAS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2383] to delta 1 Catenin/CAS (phospho T916) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293T cell lysates treated with calyculin A; human ovarian carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2383 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P |
| 特記事項 |
|---|
追加情報
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/5000 - 1/50000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/5000 - 1/50000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
組織特異性
Expressed in vascular endothelium. -
配列類似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
ドメイン
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻訳後修飾
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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別名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
画像
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Western blot - Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)All lanes : Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400) at 1/50000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 100 kDa (possible isoform) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)](https://www.abcam.co.jp/ps/products/79/ab79400/Images/ab79400-94953-anti-delta-1-catenin-cas-phospho-t916-antibody-epr2383-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)ab79400 at 1/100 dilution staining delta 1 Catenin/CAS in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)](https://www.abcam.co.jp/ps/products/79/ab79400/Images/ab79400-3-benefits-of-recombinant-antibodies.png)
Anti-delta 1 Catenin/CAS (phospho T916) antibody [EPR2383] (ab79400)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab79400 は論文での使用が確認できていません。