Anti-delta 1 Catenin/CAS (phospho S268) 抗体 [EPR2380] (ab79545)
![Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.co.jp/ps/products/79/ab79545/Images/ab79545-95095-anti-delta-1-catenin-cas-phospho-s268-antibody-epr2380-western-blot.jpg "Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2380] to delta 1 Catenin/CAS (phospho S268)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380]
delta 1 Catenin/CAS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2380] to delta 1 Catenin/CAS (phospho S268) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho S268).
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ポジティブ･コントロール
- HeLa cell lysate treated with Calyculin A. A431 cells. Human breast carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2380 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
|
| 特記事項 |
|---|
|
WB
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for Flow Cyt or IP.
ターゲット情報
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機能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
組織特異性
Expressed in vascular endothelium. -
配列類似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
ドメイン
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻訳後修飾
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1500 Human
- Omim: 601045 Human
- SwissProt: O60716 Human
- Unigene: 166011 Human
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別名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
画像
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Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)All lanes : Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.co.jp/ps/products/79/ab79545/Images/ab79545-95093-anti-delta-1-catenin-cas-phospho-s268-antibody-epr2380-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)Immunofluorescent staining of delta 1 Catenin/CAS in A431 cells using ab79545 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.co.jp/ps/products/79/ab79545/Images/ab79545-95094-anti-delta-1-catenin-cas-phospho-s268-antibody-epr2380-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)ab79545 at 1/100 dilution staining delta 1 Catenin/CAS in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)](https://www.abcam.co.jp/ps/products/79/ab79545/Images/ab79545-4-benefits-of-recombinant-antibodies.png)
Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (ab79545)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab79545 は 1 報の論文で使用されています。
- Alharatani R et al. Novel truncating mutations in CTNND1 cause a dominant craniofacial and cardiac syndrome. Hum Mol Genet 29:1900-1921 (2020). PubMed: 32196547