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リコンビナントRabMAb

Anti-DEK 抗体 [EPR11034] (ab166624)

Submit a review Submit a question References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)
  • Western blot - Anti-DEK antibody [EPR11034] (ab166624)
  • Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] (ab166624)
  • Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)
  • Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)
  • Anti-DEK antibody [EPR11034] (ab166624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11034] to DEK
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-DEK antibody [EPR11034]
    DEK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11034] to DEK

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: 293T, HeLa, Jurkat and K562 cell lysates. IHC-P: Human breast and gastric carcinoma tissues. ICC/IF: 293T cells. IP: HeLa cell lysate. Flow Cyt (intra): HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11034

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab166624の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/2000.
WB
1/1000 - 1/5000. Predicted molecular weight: 43 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
特記事項
Flow Cyt (Intra)
1/2000.
WB
1/1000 - 1/5000. Predicted molecular weight: 43 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.

ターゲット情報

  • 機能

    Involved in chromatin organization.

  • 組織特異性

    Ubiquitous. Expressed at relatively high levels.

  • 関連疾患

    A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).

  • 配列類似性

    Contains 1 SAP domain.

  • 翻訳後修飾

    Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.

  • 細胞内局在

    Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
  • Information by UniProt

  • 参照データベース

    • 別名

      • D6S231E antibody
      • Dek antibody
      • DEK gene antibody
      • DEK oncogene antibody
      • DEK oncogene DNA binding antibody
      • DEK_HUMAN antibody
      • Protein DEK antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DEK with ab166624 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-DEK antibody [EPR11034] (ab166624)
      Western blot - Anti-DEK antibody [EPR11034] (ab166624)
      All lanes : Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : Jurkat cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa

    • Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] (ab166624)
      Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] (ab166624)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DEK (red) with ab166624 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)
      Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)
      Immunofluorescence analysis of 293T cells labeling DEK with ab166624 at 1/100 dilution.
    • Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)
      Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)
      Immunoprecipitation of DEK in pellet from HeLa cell lysate using ab166624 at 1/10 dilution, followed by WB detection of DEK using as secondary an HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

      Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DEK with ab166624 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-DEK antibody [EPR11034] (ab166624)
      Anti-DEK antibody [EPR11034] (ab166624)

    参考文献 (9)

    ab166624 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab166624 は 9 報の論文で使用されています。

    • Liu B  et al. DEK modulates both expression and alternative splicing of cancer‑related genes. Oncol Rep 47:N/A (2022). PubMed: 35475534
    • Cai Y  et al. Gigantol inhibits cell proliferation and induces apoptosis by regulating DEK in non-small cell lung cancer. Exp Ther Med 22:1317 (2021). PubMed: 34630671
    • Wang J  et al. CD36 upregulates DEK transcription and promotes cell migration and invasion via GSK-3ß/ß-catenin-mediated epithelial-to-mesenchymal transition in gastric cancer. Aging (Albany NY) 13:1883-1897 (2020). PubMed: 33232276
    • Zhao T  et al. Expression of DEK in pancreatic cancer and its correlation with clinicopathological features and prognosis. J Cancer 10:911-917 (2019). PubMed: 30854097
    • Hui W  et al. MicroRNA-1292-5p inhibits cell growth, migration and invasion of gastric carcinoma by targeting DEK. Am J Cancer Res 8:1228-1238 (2018). PubMed: 30094096
    • Cifdaloz M  et al. Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1. Nat Commun 8:2249 (2017). PubMed: 29269732
    • Zhang Y  et al. The DEK oncogene activates VEGF expression and promotes tumor angiogenesis and growth in HIF-1a-dependent and -independent manners. Oncotarget 7:23740-56 (2016). Human . PubMed: 26988756
    • Wu X  et al. MiR-200a Suppresses the Proliferation and Metastasis in Pancreatic Ductal Adenocarcinoma through Downregulation of DEK Gene. Transl Oncol 9:25-31 (2016). WB, IHC . PubMed: 26947878
    • Ying G & Wu Y DEK: A novel early screening and prognostic marker for breast cancer. Mol Med Rep 12:7491-5 (2015). PubMed: 26459608

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