Anti-DEK 抗体 (ab245429)
Key features and details
- Rabbit polyclonal to DEK
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DEK antibody
DEK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DEK -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human DEK aa 225-275. The exact sequence is proprietary. NP_003463.1
Database link: P35659 -
ポジティブ･コントロール
- IP: DEK IP in HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245429 was affinity purified using an epitope specific to DEK immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245429の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in chromatin organization. -
組織特異性
Ubiquitous. Expressed at relatively high levels. -
関連疾患
A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122). -
配列類似性
Contains 1 SAP domain. -
翻訳後修飾
Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA. -
細胞内局在
Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei. - Information by UniProt
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参照データベース
- Entrez Gene: 7913 Human
- Omim: 125264 Human
- SwissProt: P35659 Human
- Unigene: 484813 Human
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別名
- D6S231E antibody
- Dek antibody
- DEK gene antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody (ab245429)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for DEK with ab245429 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-DEK antibody (ab245429)DEK was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245429 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245429 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245429 は論文での使用が確認できていません。