Anti-Dectin-1 抗体 [EPR25360-19] (BSA and Azide free) (ab300498)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)](https://www.abcam.co.jp/ps/products/300/ab300498/Images/AB300498-486453-mousespleen500.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25360-19] to Dectin-1
- Suitable for: IHC-P
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free)
Dectin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25360-19] to Dectin-1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details
適用なし: Flow Cyt (Intra),ICC/IF,IP or WB -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Mouse spleen, lung, and lung cancer tissues.
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特記事項
ab300498 is a carrier free version of ab300497.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25360-19 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300498の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
組織特異性
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
関連疾患
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
配列類似性
Contains 1 C-type lectin domain. -
翻訳後修飾
Phosphorylated on tyrosine residues in response to glucan binding. -
細胞内局在
Cytoplasm and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 56644 Mouse
- SwissProt: Q6QLQ4 Mouse
- Unigene: 239516 Mouse
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別名
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)
This data was developed using ab300497, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling Dectin-1 with ab300497 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse spleen is observed (PMID: 15107454). The section was incubated with ab300497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)](https://www.abcam.co.jp/ps/products/300/ab300498/Images/AB300498-486455-mouselung500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)This data was developed using ab300497, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labelling Dectin-1 with ab300497 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse lung is observed. The section was incubated with ab300497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)](https://www.abcam.co.jp/ps/products/300/ab300498/Images/AB300498-486457-mouselungcancer500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)This data was developed using ab300497, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labelling Dectin-1 with ab300497 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse lung cancer is observed. The section was incubated with ab300497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)](https://www.abcam.co.jp/ps/products/300/ab300498/Images/AB300498-486460-mousebrain500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)This data was developed using ab300497, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mousecerebrum tissue labelling Dectin-1 with ab300497 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: No staining on mouse cerebrum is observed. The section was incubated with ab300497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
-
![Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)](https://www.abcam.co.jp/ps/products/300/ab300498/Images/AB300498-486451-Benefits-of-Recombinant-antibodies.jpg)
Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free) (AB300498)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300498 は論文での使用が確認できていません。
