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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-DDX6 抗体 [EPR12146] (ab174277)

Reviews (1) Submit a question References (1)
Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)
  • Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] (ab174277)
  • Anti-DDX6 antibody [EPR12146] (ab174277)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12146] to DDX6
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-DDX6 antibody [EPR12146]
    DDX6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12146] to DDX6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human DDX6 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P26196

  • ポジティブ･コントロール

    • 293T, HepG2, HeLa and K562 cell lysates, Human heart tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12146

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab174277の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
特記事項
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 関連性

    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.

  • 細胞内局在

    Cytoplasm; P-body. Note: Processing bodies (PB).

  • 参照データベース

    • 別名

      • ATP dependent RNA helicase DDX6 antibody
      • ATP dependent RNA helicase p54 antibody
      • DDX6 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
      • DEAD box 6 antibody
      • DEAD box protein 6 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
      • FLJ36338 antibody
      • HLR2 antibody
      • Oncogene RCK antibody
      • P54 antibody
      • Probable ATP-dependent RNA helicase DDX6 antibody
      • RCK antibody
      see all

    画像

    • Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)
      Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: DDX6 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab174277 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab174277 was shown to recognize DDX6 when DDX6 knockout samples were used, along with additional cross-reactive bands. Wild-type and DDX6 knockout samples were subjected to SDS-PAGE. ab174277 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)
      Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)
      All lanes : Anti-DDX6 antibody [EPR12146] (ab174277) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa
      Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] (ab174277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] (ab174277)

      Immunohistochemical analysis of paraffin embedded Human heart tissue labeling DDX6 with ab174277 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-DDX6 antibody [EPR12146] (ab174277)
      Anti-DDX6 antibody [EPR12146] (ab174277)

    参考文献 (1)

    ab174277 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab174277 は 1 報の論文で使用されています。

    • D'Lima NG  et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 13:174-180 (2017). PubMed: 27918561

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-DDX6 antibody [EPR12146]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    mouse embryonic stem cells (J1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Feb 01 2018

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