Anti-DDX6 抗体 (ab70455)
Key features and details
- Rabbit polyclonal to DDX6
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX6 antibody
DDX6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDX6 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a sequence between N terminal residues 1 to 50 of human DDX6 (Swiss-Prot P26196).
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70455 was affinity purified using an epitope specific to DDX6 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70455の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB | (2) |
1/5000 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/15000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS. -
細胞内局在
Cytoplasm; P-body. Note: Processing bodies (PB). -
参照データベース
- Entrez Gene: 419783 Chicken
- Entrez Gene: 513906 Cow
- Entrez Gene: 100135517 Guinea pig
- Entrez Gene: 1656 Human
- Entrez Gene: 13209 Mouse
- Entrez Gene: 100190811 Orangutan
- Entrez Gene: 500988 Rat
- Omim: 600326 Human
see all -
別名
- ATP dependent RNA helicase DDX6 antibody
- ATP dependent RNA helicase p54 antibody
- DDX6 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (ab70455)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DDX6 with ab70455 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-DDX6 antibody (ab70455)All lanes : Anti-DDX6 antibody (ab70455) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunoprecipitation - Anti-DDX6 antibody (ab70455)250µg HeLa whole cell lysate treated with ab70455 at 0.04 µg/ml for the Western blot, and at 3 µg/mg lysate for Immunoprecipitation.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab70455 は 2 報の論文で使用されています。
- Wilczynska A et al. eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR. Genome Biol 20:262 (2019). PubMed: 31791371
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708