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遺伝子ノックアウト細胞株 検証済

Anti-DDX5 抗体 [2257C3a] (ab53216)

Submit a review Submit a question References (3)
Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
  • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
  • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
  • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)

Key features and details

  • Mouse monoclonal [2257C3a] to DDX5
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-DDX5 antibody [2257C3a]
    DDX5 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2257C3a] to DDX5

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment of human DDX5.

  • ポジティブ･コントロール

    • HeLa whole cell lysate (ab150035), NIH3T3 whole cell lysate and F2408 whole cell lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.

  • ポリ/モノ

    モノクローナル

  • クローン名

    2257C3a

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab53216の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
特記事項
WB
1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

ターゲット情報

  • 機能

    RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.

  • 配列類似性

    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • 翻訳後修飾

    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.

  • 細胞内局在

    Nucleus > nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ATP dependent RNA helicase DDX5 antibody
      • DDX 5 antibody
      • Ddx5 antibody
      • DDX5_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
      • DEAD box 5 antibody
      • DEAD box protein 5 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
      • G17P1 antibody
      • HELR antibody
      • HLR1 antibody
      • HUMP68 antibody
      • P68 antibody
      • p68 RNA helicase antibody
      • Probable ATP dependent RNA helicase DDX5 antibody
      • Probable ATP-dependent RNA helicase DDX5 antibody
      • RNA helicase p68 antibody
      see all

    画像

    • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab53216 observed at 69 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab53216 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. Ab53216 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µg

      Secondary
      anti-Mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa
      Observed band size: 69 kDa

    • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      anti-Mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa
      Observed band size: 69 kDa

    • Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)
      Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa
      Observed band size: 69 kDa

    参考文献 (3)

    ab53216 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab53216 は 3 報の論文で使用されています。

    • Xu J  et al. The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NF?B transcripts to dampen antiviral innate immunity. PLoS Pathog 17:e1009530 (2021). PubMed: 33909701
    • Pettersson OJ  et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). PubMed: 24792155
    • Yao H  et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). PubMed: 20966046

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