Anti-DDX49 抗体 (ab188010)
Key features and details
- Rabbit polyclonal to DDX49
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX49 antibody -
製品の詳細
Rabbit polyclonal to DDX49 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human DDX49 aa 350-450.
Database link: Q9Y6V7 -
ポジティブ･コントロール
- IHC-P: Human stomach tissue; WB: RT-4 and U-251 MG whole cell lysates, Human plasma (IgG/HSA depleted), Human liver and tonsil tissue lysates; ICC: U-2 OS whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab188010の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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配列類似性
Belongs to the DEAD box helicase family. DDX49/DBP8 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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参照データベース
- Entrez Gene: 54555 Human
- Entrez Gene: 234374 Mouse
- Entrez Gene: 290660 Rat
- SwissProt: Q9Y6V7 Human
- SwissProt: Q4FZF3 Mouse
- Unigene: 143187 Human
- Unigene: 388127 Mouse
- Unigene: 440659 Mouse
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別名
- DDX49 antibody
- DDX49_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 49 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX49 antibody (ab188010)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling DDX49 in the cytoplasm of glandular cells with ab188010 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-DDX49 antibody (ab188010)All lanes : Anti-DDX49 antibody (ab188010) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 54 kDa -
Immunocytochemistry - Anti-DDX49 antibody (ab188010)Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling DDX49 in the nucleoli and mitochondria with ab188010 at a 2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab188010 は 1 報の論文で使用されています。
- Awasthi S et al. DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA. Nucleic Acids Res 46:6304-6317 (2018). PubMed: 29618122