Anti-DDX47 抗体 (ab176852)
Key features and details
- Rabbit polyclonal to DDX47
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX47 antibody
DDX47 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDX47 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Gorilla, Orangutan
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免疫原
Synthetic peptide within Human DDX47 aa 1-50 (N terminal). The exact sequence is proprietary.
Sequence:MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI
Database link: Q9H0S4 -
ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176852 was affinity purified using an epitope specific to DDX47 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176852の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. |
ターゲット情報
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配列類似性
Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 101148379 Gorilla
- Entrez Gene: 51202 Human
- Entrez Gene: 100450882 Orangutan
- SwissProt: Q9H0S4 Human
- Unigene: 719938 Human
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別名
- DDX47 antibody
- DDX47_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 47 antibody
see all
画像
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Western blot - Anti-DDX47 antibody (ab176852)All lanes : Anti-DDX47 antibody (ab176852) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-DDX47 antibody (ab176852)Ab176852 immunoprecipitating DDX47 in HeLa whole cell lysate. 3µg/mg of cell lysate was incubated with primary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab176852 は 1 報の論文で使用されています。
- Mueller F et al. Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization. Sci Adv 7:N/A (2021). PubMed: 33523899
