Anti-DDX39 抗体 [2252C4a] (ab50697)
![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.co.jp/ps/products/50/ab50697/Images/ab50697-62369-anti-ddx39-antibody-2252c4a-western-blot.jpg "Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)")
Key features and details
- Mouse monoclonal [2252C4a] to DDX39
- Suitable for: WB, IP
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-DDX39 antibody [2252C4a]
DDX39 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2252C4a] to DDX39 -
由来種
Mouse -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Recombinant fragment
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ポジティブ･コントロール
- WB: HeLa, NIH3T3, F2480 whole cell lysate. IP: HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane. -
ポリ/モノ
モノクローナル -
クローン名
2252C4a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) | |
| IP |
| 特記事項 |
|---|
追加情報
Dot: Use at an assay dependent dilution.
IP: Use at 5 µg.
WB: 1/50 - 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5 µg.
WB: 1/50 - 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 10212 Human
- Entrez Gene: 68278 Mouse
- SwissProt: O00148 Human
- SwissProt: Q8VDW0 Mouse
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別名
- ATP dependent RNA helicase DDX39 antibody
- ATP-dependent RNA helicase DDX39A antibody
- BAT1 antibody
see all
画像
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Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) + immunized recombinant protein
Predicted band size: 49 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.co.jp/ps/products/50/ab50697/Images/ab50697-62368-anti-ddx39-antibody-2252c4a-western-blot.jpg)
Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa -
![Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.co.jp/ps/products/50/ab50697/Images/ab50697-62367-anti-ddx39-antibody-2252c4a-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (ab50697)500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique. -
![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.co.jp/ps/products/50/ab50697/Images/ab50697-62366-anti-ddx39-antibody-2252c4a-western-blot.jpg)
Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa -
![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.co.jp/ps/products/50/ab50697/Images/ab50697-62365-anti-ddx39-antibody-2252c4a-western-blot.jpg)
Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + F2480 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab50697 は 1 報の論文で使用されています。
- Kato M et al. DDX39 acts as a suppressor of invasion for bladder cancer. Cancer Sci 103:1363-9 (2012). PubMed: 22494014