Anti-DDX27 抗体 [EPR13147] (ab177950)
![Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (ab177950)](https://www.abcam.co.jp/ps/products/177/ab177950/Images/ab177950-206140-anti-ddx27-antibody-epr13147-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (ab177950)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13147] to DDX27
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-DDX27 antibody [EPR13147]
DDX27 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13147] to DDX27 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- HeLa, HepG2 and Jurkat whole cell lysate (ab7899); HeLa cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13147 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177950の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 89 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 89 kDa. |
|
ICC/IF
1/50 - 1/100. |
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.
ターゲット情報
-
機能
Probable ATP-dependent RNA helicase. -
配列類似性
Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 55661 Human
- Entrez Gene: 228889 Mouse
- Entrez Gene: 362274 Rat
- SwissProt: Q96GQ7 Human
- SwissProt: Q921N6 Mouse
- Unigene: 404263 Human
- Unigene: 65234 Human
- Unigene: 295031 Mouse
-
別名
- C86129 antibody
- cb843 antibody
- DDX27 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (ab177950)
Immunofluorescent analysis of HeLa cells labeling DDX27 with ab177950 at 1/50 dilution.
-
![Western blot - Anti-DDX27 antibody [EPR13147] (ab177950)](https://www.abcam.co.jp/ps/products/177/ab177950/Images/ab177950-206139-anti-ddx27-antibody-epr13147-western-blot.jpg)
Western blot - Anti-DDX27 antibody [EPR13147] (ab177950)All lanes : Anti-DDX27 antibody [EPR13147] (ab177950) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDa -
![Anti-DDX27 antibody [EPR13147] (ab177950)](https://www.abcam.co.jp/ps/products/177/ab177950/Images/ab177950-3-benefits-of-recombinant-antibodies.png)
Anti-DDX27 antibody [EPR13147] (ab177950)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab177950 は論文での使用が確認できていません。